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Canine Serum Protein Patterns Using High-Resolution Electrophoresis (HRE)

ABATE, O. ; ZANATTA, R. ; MALISANO, T. ; DOTTA, U.

The veterinary journal (1997), 2000-03, Vol.159 (2), p.154-160 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Canine Serum Protein Patterns Using High-Resolution Electrophoresis (HRE)
Autor: ABATE, O. ; ZANATTA, R. ; MALISANO, T. ; DOTTA, U.
Assuntos: Animals ; Blood Protein Electrophoresis - methods ; Blood Protein Electrophoresis - veterinary ; Blood Proteins - analysis ; dog ; Dog Diseases - blood ; Dogs - blood ; electrophoresis ; Electrophoresis, Agar Gel - methods ; Electrophoresis, Agar Gel - veterinary ; Female ; Fibrinogen - analysis ; Haptoglobins - analysis ; HRE ; Lipoproteins - blood ; Male ; Reference Values ; Serum proteins
É parte de: The veterinary journal (1997), 2000-03, Vol.159 (2), p.154-160
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Serum protein values were determined in 26 healthy dogs using agarose gel electrophoresis (SPE), splitting the electrophoretic separation into six regions: albumin, α 1, α 2, β 1, β 2and γ globulins. High-resolution electrophoresis (HRE) was used to separate single proteins. Serum proteins from dogs (26 healthy and 20 affected by various diseases) were then characterized by electrophoretic immunofixation (IFE) and Sudan black staining on HRE film. Haemoglobin and normal canine plasma and serum were used to identify haptoglobin and fibrinogen, respectively. In the standard pattern, determined by HRE, the following proteins were identified: albumin, α 1-lipoprotein ( α 1-region), haptoglobin and α 2 -macroglobulin ( α 2-region), β -lipoprotein and C3 ( β 1-region), transferrin and IgM ( β 2-region), IgG (mostly in γ -region and partly in β 2-region). The HRE pattern shown by healthy dogs could be compared with those of dogs affected by various diseases to obtain clinical information.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Canine Serum Protein Patterns Using High-Resolution Electrophoresis (HRE)

ABATE, O. ; ZANATTA, R. ; MALISANO, T. ; DOTTA, U.

England: Elsevier Ltd

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