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Spatially Distinct Pools of TORC1 Balance Protein Homeostasis

Hatakeyama, Riko ; Péli-Gulli, Marie-Pierre ; Hu, Zehan ; Jaquenoud, Malika ; Garcia Osuna, Guillermo Miguel ; Sardu, Alessandro ; Dengjel, Jörn ; De Virgilio, Claudio

Molecular cell, 2019-01, Vol.73 (2), p.325-338.e8 [Periódico revisado por pares]

United States: Elsevier Inc

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Spatially Distinct Pools of TORC1 Balance Protein Homeostasis
Autor: Hatakeyama, Riko ; Péli-Gulli, Marie-Pierre ; Hu, Zehan ; Jaquenoud, Malika ; Garcia Osuna, Guillermo Miguel ; Sardu, Alessandro ; Dengjel, Jörn ; De Virgilio, Claudio
Assuntos: Adaptor Proteins, Signal Transducing - genetics ; Adaptor Proteins, Signal Transducing - metabolism ; amino acid signaling ; Atg13 ; Autophagy ; Autophagy-Related Proteins - genetics ; Autophagy-Related Proteins - metabolism ; endosomal sorting complex required for transport ; Endosomal Sorting Complexes Required for Transport - genetics ; Endosomal Sorting Complexes Required for Transport - metabolism ; Endosomes - enzymology ; Endosomes - genetics ; ESCRT ; Gene Expression Regulation, Fungal ; growth control ; macroautophagy ; microautophagy ; protein homeostasis ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - metabolism ; Proteolysis ; Proteostasis ; Rag GTPases ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Signal Transduction ; target of rapamycin complex 1 ; TORC1 ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Vacuoles - enzymology ; Vacuoles - genetics ; Vps27
É parte de: Molecular cell, 2019-01, Vol.73 (2), p.325-338.e8
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
Descrição: The eukaryotic TORC1 kinase is a homeostatic controller of growth that integrates nutritional cues and mediates signals primarily from the surface of lysosomes or vacuoles. Amino acids activate TORC1 via the Rag GTPases that combine into structurally conserved multi-protein complexes such as the EGO complex (EGOC) in yeast. Here we show that Ego1, which mediates membrane-anchoring of EGOC via lipid modifications that it acquires while traveling through the trans-Golgi network, is separately sorted to vacuoles and perivacuolar endosomes. At both surfaces, it assembles EGOCs, which regulate spatially distinct pools of TORC1 that impinge on functionally divergent effectors: vacuolar TORC1 predominantly targets Sch9 to promote protein synthesis, whereas endosomal TORC1 phosphorylates Atg13 and Vps27 to inhibit macroautophagy and ESCRT-driven microautophagy, respectively. Thus, the coordination of three key regulatory nodes in protein synthesis and degradation critically relies on a division of labor between spatially sequestered populations of TORC1. [Display omitted] •Rag GTPases and TORC1 assemble at the surfaces of both endosomes and vacuoles•These spatially and functionally distinct pools of TORC1 target specific effectors•Vacuolar TORC1 promotes protein synthesis through its proximal effector Sch9•Endosomal TORC1 curbs macro- and microautophagy via Atg13 and Vps27, respectively The Rag GTPase-TORC1 signaling module integrates amino acid signals to control eukaryotic growth in a homeostatic manner. Here, Hatakeyama et al. show that functionally autonomous pools of these modules assemble at vacuolar and endosomal membranes to balance spatially distinct key regulatory nodes in protein synthesis and degradation, respectively.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Spatially Distinct Pools of TORC1 Balance Protein Homeostasis

Hatakeyama, Riko ; Péli-Gulli, Marie-Pierre ; Hu, Zehan ; Jaquenoud, Malika ; Garcia Osuna, Guillermo Miguel ; Sardu, Alessandro ; Dengjel, Jörn ; De Virgilio, Claudio

United States: Elsevier Inc

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