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Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures

Cho, Hwa-Young ; Son, Seok Young ; Rhee, Hong Soon ; Yoon, Sung-Yong H. ; Lee-Parsons, Carolyn W.T. ; Park, Jong Moon

Journal of biotechnology, 2008-05, Vol.135 (1), p.117-122 [Periódico revisado por pares]

Lausanne: Elsevier B.V

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Título:
Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures
Autor: Cho, Hwa-Young ; Son, Seok Young ; Rhee, Hong Soon ; Yoon, Sung-Yong H. ; Lee-Parsons, Carolyn W.T. ; Park, Jong Moon
Assuntos: ( S)-Methylcoclaurine-3′-hydroxylase ; 3′-Hydroxy-(S)- N-methylcoclaurine-4′- O-methyltransferase ; Acetates - administration & dosage ; alkaloids ; Alkaloids - metabolism ; application timing ; Benzophenanthridine alkaloid ; benzophenanthridine alkaloids ; Benzophenanthridines - metabolism ; Biological and medical sciences ; biosynthesis ; Biotechnology ; Cell Extracts - administration & dosage ; Cell Extracts - chemistry ; cell suspension culture ; Cells, Cultured ; Cyclopentanes - administration & dosage ; Dose-Response Relationship, Drug ; Drug Combinations ; Drug Synergism ; elicitors ; enzyme activity ; Eschscholtzia californica ; Eschscholzia - drug effects ; Eschscholzia - metabolism ; Eschscholzia californica ; Fundamental and applied biological sciences. Psychology ; Methyl jasmonate ; Oxylipins - administration & dosage ; Plant Proteins - metabolism ; protein synthesis ; Salicylic acid ; Salicylic Acid - administration & dosage ; Signal transduction ; Yeast extract ; Yeasts - chemistry
É parte de: Journal of biotechnology, 2008-05, Vol.135 (1), p.117-122
Notas: http://dx.doi.org/10.1016/j.jbiotec.2008.02.020
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SourceType-Scholarly Journals-1
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Descrição: To develop an optimal bioprocess for secondary metabolite production and explain the bioprocess at the molecular level, we examine the synergistic effects of sequential treatment with methyl jasmonate (MJ), salicylic acid (SA) and yeast extract (YE) on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures. Serial treatment of MJ, SA and YE at 24 h intervals enhanced the accumulation of dihydrosanguinarine (2.5 times) and sanguinarine (5.5 times). This sequential treatment using different signal elicitors was more effective than single elicitor or simultaneous treatment of the elicitors; it induced benzophenanthridine alkaloid accumulation to 917.7 ± 42.0 mg/L. Also, ( S)-methylcoclaurine-3′-hydroxylase (CYP80B1) and 3′-hydroxy-(S)- N-methylcoclaurine-4′- O-methyltransferase (4′OMT) expressions among enzymes in sanguinarine biosynthetic pathway explained the synergistic effects by sequential treatment of the elicitors. The sequential treatment strategy using elicitors related to different signal transduction pathways can be used to design better processes to increase accumulation of secondary metabolites in plant cell culture. Analysis of protein expression provides the detailed information about metabolite accumulation through the correlated results.
Editor: Lausanne: Elsevier B.V
Idioma: Inglês

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Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures

Cho, Hwa-Young ; Son, Seok Young ; Rhee, Hong Soon ; Yoon, Sung-Yong H. ; Lee-Parsons, Carolyn W.T. ; Park, Jong Moon

Lausanne: Elsevier B.V

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