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CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance

Li, Rui ; Liu, Chunxue ; Zhao, Ruirui ; Wang, Liu ; Chen, Lin ; Yu, Wenqing ; Zhang, Shujuan ; Sheng, Jiping ; Shen, Lin

BMC plant biology, 2019-01, Vol.19 (1), p.38-38, Article 38 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance
Autor: Li, Rui ; Liu, Chunxue ; Zhao, Ruirui ; Wang, Liu ; Chen, Lin ; Yu, Wenqing ; Zhang, Shujuan ; Sheng, Jiping ; Shen, Lin
Assuntos: Abiotic stress ; Adaptation, Physiological - genetics ; Agricultural production ; Antioxidants ; Apertures ; CRISPR ; CRISPR-Cas Systems ; CRISPR/Cas9 ; Drought ; Drought resistance ; Droughts ; Economic models ; Environmental aspects ; Enzymes ; Gene expression ; Gene mutations ; Genes, Plant ; Genetic aspects ; Genomics ; Hardiness ; Hydrogen peroxide ; Identification and classification ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - physiology ; Malondialdehyde ; Mutagenesis ; Mutants ; Oxidative stress ; Pathogenesis ; Pattern analysis ; Physiological aspects ; Plant growth ; Plants ; Proteins ; Regulatory mechanisms (biology) ; Rice ; ROS ; SlNPR1 ; Stomata ; Stomatal closure ; Stress, Physiological - genetics ; Tobacco ; Tomato plant ; Tomatoes
É parte de: BMC plant biology, 2019-01, Vol.19 (1), p.38-38, Article 38
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: NPR1, nonexpressor of pathogenesis-related gene 1, is a master regulator involved in plant defense response to pathogens, and its regulatory mechanism in the defense pathway has been relatively clear. However, information about the function of NPR1 in plant response to abiotic stress is still limited. Tomato is the fourth most economically crop worldwide and also one of the best-characterized model plants employed in genetic studies. Because of the lack of a stable tomato NPR1 (SlNPR1) mutant, little is known about the function of SlNPR1 in tomato response to biotic and abiotic stresses. Here we isolated SlNPR1 from tomato 'Ailsa Craig' and generated slnpr1 mutants using the CRISPR/Cas9 system. Analysis of the cis-acting elements indicated that SlNPR1 might be involved in tomato plant response to drought stress. Expression pattern analysis showed that SlNPR1 was expressed in all plant tissues, and it was strongly induced by drought stress. Thus, we investigated the function of SlNPR1 in tomato-plant drought tolerance. Results showed that slnpr1 mutants exhibited reduced drought tolerance with increased stomatal aperture, higher electrolytic leakage, malondialdehyde (MDA) and hydrogen peroxide (H O ) levels, and lower activity levels of antioxidant enzymes, compared to wild type (WT) plants. The reduced drought tolerance of slnpr1 mutants was further reflected by the down-regulated expression of drought related key genes, including SlGST, SlDHN, and SlDREB. Collectively, the data suggest that SlNPR1 is involved in regulating tomato plant drought response. These results aid in further understanding the molecular basis underlying SlNPR1 mediation of tomato drought sensitivity.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance

Li, Rui ; Liu, Chunxue ; Zhao, Ruirui ; Wang, Liu ; Chen, Lin ; Yu, Wenqing ; Zhang, Shujuan ; Sheng, Jiping ; Shen, Lin

England: BioMed Central Ltd

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