skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

Antisense inhibition of gene expression in cells by oligonucleotides incorporating locked nucleic acids: effect of mRNA target sequence and chimera design

Braasch, Dwaine A. ; Liu, Yinghui ; Corey, David R.

Nucleic acids research, 2002-12, Vol.30 (23), p.5160-5167 [Periódico revisado por pares]

England: Oxford University Press

Texto completo disponível

Citações Citado por
  • Título:
    Antisense inhibition of gene expression in cells by oligonucleotides incorporating locked nucleic acids: effect of mRNA target sequence and chimera design
  • Autor: Braasch, Dwaine A. ; Liu, Yinghui ; Corey, David R.
  • Assuntos: 5' Untranslated Regions ; Animals ; Binding Sites ; Cell Line ; Codon, Initiator ; Dose-Response Relationship, Drug ; Drug Delivery Systems ; Drug Design ; Gene Expression Regulation ; Luciferases - biosynthesis ; Luciferases - genetics ; Nucleic Acid Denaturation ; Nucleic Acid Hybridization ; Oligonucleotides, Antisense - chemistry ; Oligonucleotides, Antisense - pharmacology ; Protein Biosynthesis ; RNA, Messenger - chemistry ; RNA, Messenger - metabolism ; Temperature ; Transfection
  • É parte de: Nucleic acids research, 2002-12, Vol.30 (23), p.5160-5167
  • Notas: Received August 16, 2002; Revised and Accepted September 25, 2002
    local:gkf651
    istex:28CB763E6EBAE8DC2ADC6FD761124A64A88E2D7F
    ark:/67375/HXZ-590PXMDN-K
    To whom correspondence should be addressed. Tel: +1 214 648 5096; Fax: +1 214 648 5095; Email: david.corey@utsouthwestern.edu
 The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
    ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-1
    content type line 23
    ObjectType-Article-1
    ObjectType-Feature-2
    To whom correspondence should be addressed. Tel: +1 214 648 5096; Fax: +1 214 648 5095; Email: david.corey@utsouthwestern.edu The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
  • Descrição: Use of antisense oligonucleotides is a versatile strategy for achieving control of gene expression. Unfortunately, the interpretation of antisense‐induced phenotypes is sometimes difficult, and chemical modifications that improve the potency and specificity of antisense action would be useful. The introduction of locked nucleic acid (LNA) bases into oligonucleotides confers exceptional improvement in binding affinity, up to 10°C per substitution, making LNAs an exciting option for the optimization of antisense efficacy. Here we examine the rules governing antisense gene inhibition within cells by oligonucleotides that contain LNA bases. LNA‐ containing oligomers were transfected into cells using cationic lipid and accumulated in the nucleus. We tested antisense gene inhibition by LNAs and LNA–DNA chimeras complementary to the 5′‐untranslated region, the region surrounding the start codon and the coding region of mRNA, and identified effective antisense agents targeted to each of these locations. Our data suggest that LNA bases can be used to develop antisense oligonucleotides and that their use is a versatile approach for efficiently inhibiting gene expression inside cells.
  • Editor: England: Oxford University Press
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.