Idioma:

Myocardial Regeneration by Activation of Multipotent Cardiac Stem Cells in Ischemic Heart Failure

Urbanek, Konrad ; Torella, Daniele ; Sheikh, Farooq ; De Angelis, Antonella ; Nurzynska, Daria ; Silvestri, Furio ; Beltrami, C. Alberto ; Bussani, Rossana ; Beltrami, Antonio P. ; Quaini, Federico ; Bolli, Roberto ; Leri, Annarosa ; Kajstura, Jan ; Anversa, Piero ; Ignarro, Louis J.

Proceedings of the National Academy of Sciences - PNAS, 2005-06, Vol.102 (24), p.8692-8697 [Periódico revisado por pares]

United States: National Academy of Sciences

Texto completo disponível

Citações Citado por

Enviar para

Título:
Myocardial Regeneration by Activation of Multipotent Cardiac Stem Cells in Ischemic Heart Failure
Autor: Urbanek, Konrad ; Torella, Daniele ; Sheikh, Farooq ; De Angelis, Antonella ; Nurzynska, Daria ; Silvestri, Furio ; Beltrami, C. Alberto ; Bussani, Rossana ; Beltrami, Antonio P. ; Quaini, Federico ; Bolli, Roberto ; Leri, Annarosa ; Kajstura, Jan ; Anversa, Piero ; Ignarro, Louis J.
Assuntos: Apoptosis ; Apoptosis - physiology ; Biological Sciences ; Blotting, Western ; Bone marrow ; Cell Differentiation - physiology ; Cellular senescence ; Cyclin-Dependent Kinase Inhibitor p16 - metabolism ; DNA-Binding Proteins ; Endothelial cells ; Heart ; Heart - physiology ; Heart attacks ; Heart failure ; Humans ; In Situ Hybridization, Fluorescence ; Medical research ; Mitosis - physiology ; Multipotent stem cells ; Multipotent Stem Cells - cytology ; Multipotent Stem Cells - physiology ; Myocardial Infarction - physiopathology ; Myocardium ; Myocardium - cytology ; Progenitor cells ; Regeneration - physiology ; Stem cells ; Telomerase - metabolism ; Telomere - physiology ; Telomeres
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2005-06, Vol.102 (24), p.8692-8697
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
This paper was submitted directly (Track II) to the PNAS office.
Edited by Louis J. Ignarro, University of California School of Medicine, Los Angeles, CA, and approved April 11, 2005
To whom correspondence should be addressed. E-mail: piero_anversa@nymc.edu.
Author contributions: K.U., R. Bolli, A.L., J.K., and P.A. designed research; K.U., D.T., F. Sheikh, A.D.A., D.N., F. Silvestri, C.A.B., R. Bussani, A.P.B., F.Q., A.L., and J.K. performed research; K.U., D.T., F. Sheikh, A.D.A., D.N., F. Silvestri, C.A.B., R. Bussani, A.P.B., F.Q., R. Bolli, A.L., J.K., and P.A. analyzed data; and K.U., F.Q., R. Bolli, A.L., J.K., and P.A. wrote the paper.
Abbreviations: CSC, cardiac stem cell; EC, endothelial cell; MI, myocardial infarction; SMC, smooth muscle cell; TERT, telomerase.
Descrição: In this study, we tested whether the human heart possesses a cardiac stem cell (CSC) pool that promotes regeneration after infarction. For this purpose, CSC growth and senescence were measured in 20 hearts with acute infarcts, 20 hearts with end-stage postinfarction cardiomyopathy, and 12 control hearts. CSC number increased markedly in acute and, to a lesser extent, in chronic infarcts. CSC growth correlated with the increase in telomerase-competent dividing CSCs from 1.5% in controls to 28% in acute infarcts and 14% in chronic infarcts. The CSC mitotic index increased 29-fold in acute and 14-fold in chronic infarcts. CSCs committed to the myocyte, smooth muscle, and endothelial cell lineages increased ≈85-fold in acute infarcts and ≈25-fold in chronic infarcts. However, p16INK4 a- p53-positive senescent CSCs also increased and were 10%, 18%, and 40% in controls, acute infarcts, and chronic infarcts, respectively. Old CSCs had short telomeres and apoptosis involved 0.3%, 3.8%, and 9.6% of CSCs in controls, acute infarcts, and chronic infarcts, respectively. These variables reduced the number of functionally competent CSCs from ≈ 26,000/ cm3of viable myocardium in acute to ≈ 7,000/ cm3in chronic infarcts, respectively. In seven acute infarcts, foci of spontaneous myocardial regeneration that did not involve cell fusion were identified. In conclusion, the human heart possesses a CSC compartment, and CSC activation occurs in response to ischemic injury. The loss of functionally competent CSCs in chronic ischemic cardiomyopathy may underlie the progressive functional deterioration and the onset of terminal failure.
Editor: United States: National Academy of Sciences
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Myocardial Regeneration by Activation of Multipotent Cardiac Stem Cells in Ischemic Heart Failure

Urbanek, Konrad ; Torella, Daniele ; Sheikh, Farooq ; De Angelis, Antonella ; Nurzynska, Daria ; Silvestri, Furio ; Beltrami, C. Alberto ; Bussani, Rossana ; Beltrami, Antonio P. ; Quaini, Federico ; Bolli, Roberto ; Leri, Annarosa ; Kajstura, Jan ; Anversa, Piero ; Ignarro, Louis J.

United States: National Academy of Sciences

Buscando em bases de dados remotas. Favor aguardar.