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Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

Klapper, Paul E. ; Cleator, Graham M. ; Dennett, Carol ; Lewis, Alfor G.

Journal of medical virology, 1990-12, Vol.32 (4), p.261-264 [Periódico revisado por pares]

New York: Wiley Subscription Services, Inc., A Wiley Company

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Título:
Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction
Autor: Klapper, Paul E. ; Cleator, Graham M. ; Dennett, Carol ; Lewis, Alfor G.
Assuntos: Base Sequence ; Biological and medical sciences ; Blotting, Southern ; DNA, Viral - cerebrospinal fluid ; Encephalitis - complications ; Encephalitis - diagnosis ; Fundamental and applied biological sciences. Psychology ; Herpes Simplex - complications ; Herpes Simplex - diagnosis ; herpes simplex virus ; Humans ; Microbiology ; Molecular Sequence Data ; non-invasive diagnosis ; Polymerase Chain Reaction ; Simplexvirus - enzymology ; Simplexvirus - genetics ; Techniques used in virology ; thymidine kinase ; Thymidine Kinase - genetics ; Virology
É parte de: Journal of medical virology, 1990-12, Vol.32 (4), p.261-264
Notas: ArticleID:JMV1890320413
istex:58EE6FF0832ACE50BCAD327E908F7B11C51CF042
ark:/67375/WNG-QZKFNSPF-J
Carl Barnett Encephalitis Fund
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
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Descrição: Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumber cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR‐SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant “non‐invasive” method for the diagnosis of HSE.
Editor: New York: Wiley Subscription Services, Inc., A Wiley Company
Idioma: Inglês

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Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

Klapper, Paul E. ; Cleator, Graham M. ; Dennett, Carol ; Lewis, Alfor G.

New York: Wiley Subscription Services, Inc., A Wiley Company

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