skip to main content
Idioma:

Evaluation of the concordance of immunological biomarkers between core biopsy and corresponding resection specimen in ER/PR negative breast cancer

Vos, Hanne ; Lambein, Kathleen ; Nevelsteen, Ines ; Laenen, Annouschka ; Floris, Beppe ; Smeets, Ann

Elsevier 2018

Texto completo disponível

Citações Citado por
  • Título:
    Evaluation of the concordance of immunological biomarkers between core biopsy and corresponding resection specimen in ER/PR negative breast cancer
  • Autor: Vos, Hanne ; Lambein, Kathleen ; Nevelsteen, Ines ; Laenen, Annouschka ; Floris, Beppe ; Smeets, Ann
  • Assuntos: Breast Cancer ; Corebiopsy ; Immunotherapy
  • Notas: European Breast Cancer Conference location:Barcelona date:21 Mar - 23 Mar 2018
    EUROPEAN JOURNAL OF CANCER vol:92 pages:S134-S134
  • Descrição: Background: In breast cancer, a diagnostic core needle biopsy is routinely used to determine tumour characteristics such as histological type, grade and predictive markers. A good concordance between these characteristics on core biopsy and resection specimen has been shown. However, evidence for a similar concordance with immunological markers is still lacking. Nevertheless, response to therapy in neo-adjuvant clinical trials with immunotherapy is often determined by comparing immunological markers on core biopsy and resection specimen. Therefore, the aim of this retrospective study was to evaluate the concordance between a panel of immunological markers on core biopsy and the corresponding resection specimen. Material and methods: The core biopsy and matching resection specimen were analysed for 35 patients who underwent primary surgery for an early ER/PR negative invasive ductal adenocarcinoma of at least 2 cm. Haematoxylin-eosin staining was performed to determine the percentage of tumour-infiltrating lymphocytes (TILs). The expression of CD3, CD4, CD8, CD68, CD73, FoxP3 and Ki67 were assessed semi-quantitatively in a subgroup of 14 patients using immunohistochemistry. Statistical analysis was performed to calculate the concordance and intraclass correlation coefficient (ICC) between core biopsy and resection specimen. Results: A positive association was found between core biopsy and resection specimen for the following variables: TILs, CD3, CD8 and Ki67. However, there was a systematic difference between both measurements, with a higher average value on the resection specimen. The ICC showed a fair agreement for the TILs and Ki67 scores and a poor agreement for CD3 and CD8 scores. No association was found between CD68 measured at both time points. There was a systematic difference for CD68 between both measurements, with a higher average value at resection. No evidence could be found for an association between CD73 measured at both time points. Nonetheless, there is no statistical evidence for a systematic difference between both measurements. Statistical analysis could not be performed for CD4 and FoxP3 due to a lack of measured variability. Conclusions: These preliminary results show a fair concordance between the core biopsy and the corresponding resection specimen for TILs and Ki67 scoring. Only poor or no concordance was observed for the other immunological markers: CD3, CD8, CD68 and CD73. Our current dataset will be expanded in the next months to corroborate these preliminary results. These findings shed new light on the predictive or prognostic use of immunological biomarkers measured in core biopsies. status: published
  • Editor: Elsevier
  • Data de criação/publicação: 2018
  • Idioma: Inglês
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.