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Na⁺ transporter, TaHKT1;5‐D, limits shoot Na⁺ accumulation in bread wheat

Byrt, Caitlin Siobhan ; Xu, Bo ; Krishnan, Mahima ; Lightfoot, Damien James ; Athman, Asmini ; Jacobs, Andrew Keith ; Watson‐Haigh, Nathan S ; Plett, Darren ; Munns, Rana ; Tester, Mark ; Gilliham, Matthew

The Plant journal : for cell and molecular biology, 2014-11, Vol.80 (3), p.516-526 [Periódico revisado por pares]

England: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology

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Título:
Na⁺ transporter, TaHKT1;5‐D, limits shoot Na⁺ accumulation in bread wheat
Autor: Byrt, Caitlin Siobhan ; Xu, Bo ; Krishnan, Mahima ; Lightfoot, Damien James ; Athman, Asmini ; Jacobs, Andrew Keith ; Watson‐Haigh, Nathan S ; Plett, Darren ; Munns, Rana ; Tester, Mark ; Gilliham, Matthew
Assuntos: Animals ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis thaliana ; Base Sequence ; Bread ; Cation Transport Proteins - genetics ; Cation Transport Proteins - metabolism ; Crop science ; DNA ; Gene Expression ; Gene Expression Regulation, Plant ; Genes ; genetically modified organisms ; Genomics ; HKT ; leaves ; loci ; mesophyll ; Molecular Sequence Data ; Oocytes ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - genetics ; Plant Roots - metabolism ; plasma membrane ; polymerase chain reaction ; potassium ; protoplasts ; RNA ; RNA interference ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Salinity ; Salt-Tolerance ; Sequence Analysis, DNA ; shoots ; sodium ; Sodium - metabolism ; sodium exclusion ; stele ; Symporters - genetics ; Symporters - metabolism ; Transgenes ; Triticum - cytology ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum ; Wheat ; Xenopus laevis ; Xylem - metabolism ; xylem vessels
É parte de: The Plant journal : for cell and molecular biology, 2014-11, Vol.80 (3), p.516-526
Notas: http://dx.doi.org/10.1111/tpj.12651
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SourceType-Scholarly Journals-1
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Descrição: Bread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K⁺/Na⁺ ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we decipher the function of TaHKT1;5‐D, a candidate gene underlying the Kna1 locus. Transport studies using the heterologous expression systems Saccharomyces cerevisiae and Xenopus laevis oocytes indicated that TaHKT1;5‐D is a Na⁺‐selective transporter. Transient expression in Arabidopsis thaliana mesophyll protoplasts and in situ polymerase chain reaction indicated that TaHKT1;5‐D is localised on the plasma membrane in the wheat root stele. RNA interference‐induced silencing decreased the expression of TaHKT1;5‐D in transgenic bread wheat lines which led to an increase in the Na⁺ concentration in the leaves. This indicates that TaHKT1;5‐D retrieves Na⁺ from the xylem vessels in the root and has an important role in restricting the transport of Na⁺ from the root to the leaves in bread wheat. Thus, TaHKT1;5‐D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na⁺ exclusion and is critical in maintaining a high K⁺/Na⁺ ratio in the leaves. These findings show there is potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes.
Editor: England: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology
Idioma: Inglês

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Na⁺ transporter, TaHKT1;5‐D, limits shoot Na⁺ accumulation in bread wheat

Byrt, Caitlin Siobhan ; Xu, Bo ; Krishnan, Mahima ; Lightfoot, Damien James ; Athman, Asmini ; Jacobs, Andrew Keith ; Watson‐Haigh, Nathan S ; Plett, Darren ; Munns, Rana ; Tester, Mark ; Gilliham, Matthew

England: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology

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