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Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase

Roxas, V.P. (Texas Tech Univ., Lubbock (USA)) ; Lodhi, S.A ; Garrett, D.K ; Mahan, J.R ; Allen, R.D

Plant and cell physiology, 2000-11, Vol.41 (11), p.1229-1234 [Periódico revisado por pares]

Japan: Oxford University Press

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Título:
Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase
Autor: Roxas, V.P. (Texas Tech Univ., Lubbock (USA)) ; Lodhi, S.A ; Garrett, D.K ; Mahan, J.R ; Allen, R.D
Assuntos: Adaptation, Physiological - genetics ; APX ; ASA ; ascorbate peroxidase ; ascorbic acid ; Ascorbic Acid - metabolism ; days post-imbibition ; dehydroascorbate ; dehydroascorbate reductase ; DHA ; DHAR ; DPI ; ENZYMIC ACTIVITY ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Genotype ; GLUTATHIONE ; Glutathione - metabolism ; GLUTATHIONE PEROXIDASE ; Glutathione Peroxidase - genetics ; Glutathione Peroxidase - metabolism ; glutathione reductase ; glutathione S-transferase ; Glutathione Transferase - genetics ; Glutathione Transferase - metabolism ; GPX glutathione peroxidase ; GROWTH ; GSH ; GSSG ; GST ; Key words: Glutathione peroxidase — Glutathione S-transferase — Seedling growth — Stress tolerance — Transgenic tobacco ; Lipid Peroxidation ; malondialdehyde ; MDA ; MDHA ; MDHAR ; monodehydroascorbate ; monodehydroascorbate reductase ; Nicotiana - genetics ; Nicotiana - growth & development ; NICOTIANA TABACUM ; Oxidation-Reduction ; oxidized glutathione ; Plant Development ; Plants - genetics ; Plants, Genetically Modified ; Plants, Toxic ; reduced glutathione ; RESISTANCE TO INJURIOUS FACTORS ; SEEDLINGS ; Temperature ; TRANSFERASES ; TRANSGENIC PLANTS
É parte de: Plant and cell physiology, 2000-11, Vol.41 (11), p.1229-1234
Notas: 2001002691
F60
local:pcd051
istex:A3370BC950D4E8AB3EA161F556E501921B927714
(Received May 29, 2000; Accepted August 18, 2000).
ark:/67375/HXZ-V8K71CLN-Q
Descrição: Overexpression of a tobacco glutathione S-transferase with glutathione peroxidase activity (GST/GPX) in transgenic tobacco (Nicotiana tabacum L.) enhanced seedling growth under a variety of stressful conditions. In addition to increased GST and GPX activity, transgenic GST/GPX-expressing (GST+) seedlings had elevated levels of monodehydroascorbate reductase activity. GST+ seedlings also contained higher levels of glutathione and ascorbate than wild-type seedlings and the glutathione pools were more oxidized. Thermal or salt-stress treatments that inhibited the growth of wild-type seedlings also caused increased levels of lipid peroxidation. These treatments had less effect on the growth of GST+ seedling growth and did not lead to increased lipid peroxidation. Stress-induced damage resulted in reduced metabolic activity in wild-type seedlings while GST+ seedlings maintained metabolic activity levels comparable to seedlings grown under control conditions. These results indicate that overexpression of GST/GPX in transgenic tobacco seedlings provides increased glutathione-dependent peroxide scavenging and alterations in glutathione and ascorbate metabolism that lead to reduced oxidative damage. We conclude that this protective effect is primarily responsible for the ability of GST+ seedlings to maintain growth under stressful conditions.
Editor: Japan: Oxford University Press
Idioma: Inglês

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Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase

Roxas, V.P. (Texas Tech Univ., Lubbock (USA)) ; Lodhi, S.A ; Garrett, D.K ; Mahan, J.R ; Allen, R.D

Japan: Oxford University Press

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