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A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?

Sidhom, Karim ; Obi, Patience O ; Saleem, Ayesha

International journal of molecular sciences, 2020-09, Vol.21 (18), p.6466 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?
Autor: Sidhom, Karim ; Obi, Patience O ; Saleem, Ayesha
Assuntos: Adipose tissue ; Animals ; Cell culture ; Cell interactions ; Cell signaling ; Chromatography ; Chromatography, Gel - methods ; Communication ; Comparative studies ; Culture media ; Deoxyribonucleic acid ; Differential media ; differential ultracentrifugation ; DNA ; Ethylene glycol ; Exosomes ; Exosomes - chemistry ; Exosomes - metabolism ; extracellular vesicles ; Extracellular Vesicles - chemistry ; Extracellular Vesicles - metabolism ; Humans ; immunoaffinity capture ; Membranes ; Microfluidics ; MicroRNAs ; microvesicles ; Milk ; Mitochondrial DNA ; Physical characteristics ; Physical properties ; poly-ethylene glycol ; Polyethylene glycol ; Pore size ; Proteins ; Review ; Sedimentation & deposition ; Size exclusion chromatography ; Ultracentrifugation ; Ultracentrifugation - methods ; Ultrafiltration ; Ultrafiltration - methods ; Vesicles
É parte de: International journal of molecular sciences, 2020-09, Vol.21 (18), p.6466
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
ObjectType-Review-1
These authors contributed equally to this work.
Descrição: Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication. EVs are classified into several subtypes based on their origin, physical characteristics, and biomolecular makeup. Exosomes, a subtype of EVs, are released by the fusion of multivesicular bodies (MVB) with the plasma membrane of the cell. Several methods have been described in literature to isolate exosomes from biofluids including blood, urine, milk, and cell culture media, among others. While differential ultracentrifugation (dUC) has been widely used to isolate exosomes, other techniques including ultrafiltration, precipitating agents such as poly-ethylene glycol (PEG), immunoaffinity capture, microfluidics, and size-exclusion chromatography (SEC) have emerged as credible alternatives with pros and cons associated with each. In this review, we provide a summary of commonly used exosomal isolation techniques with a focus on SEC as an ideal methodology. We evaluate the efficacy of SEC to isolate exosomes from an array of biological fluids, with a particular focus on its application to adipose tissue-derived exosomes. We argue that exosomes isolated via SEC are relatively pure and functional, and that this methodology is reproducible, scalable, inexpensive, and does not require specialized equipment or user expertise. However, it must be noted that while SEC is a good candidate method to isolate exosomes, direct comparative studies are required to support this conclusion.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?

Sidhom, Karim ; Obi, Patience O ; Saleem, Ayesha

Switzerland: MDPI AG

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