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Estimation of skeletal muscle mass in 4-year-old children using the D3-creatine dilution method

Sidiqi, Aysha ; Fariha, Farzana ; Shanta, Shaila S ; Dasiewicz, Alison ; Mahmud, Abdullah Al ; Moore, Daniel R ; Shankaran, Mahalakshmi ; Hellerstein, Marc K ; Evans, William J ; Gernand, Alison D ; Islam, M Munirul ; Abrams, Steven A ; Harrington, Jennifer ; Nyangau, Edna ; Roth, Daniel E ; O’Callaghan, Karen M

Pediatric Research, 2023-09, Vol.94 (3), p.1195-1202

eScholarship, University of California

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  • Título:
    Estimation of skeletal muscle mass in 4-year-old children using the D3-creatine dilution method
  • Autor: Sidiqi, Aysha ; Fariha, Farzana ; Shanta, Shaila S ; Dasiewicz, Alison ; Mahmud, Abdullah Al ; Moore, Daniel R ; Shankaran, Mahalakshmi ; Hellerstein, Marc K ; Evans, William J ; Gernand, Alison D ; Islam, M Munirul ; Abrams, Steven A ; Harrington, Jennifer ; Nyangau, Edna ; Roth, Daniel E ; O’Callaghan, Karen M
  • Assuntos: Absorptiometry ; Bangladesh ; Body Composition ; Child ; Clinical Research ; Creatine ; Creatinine ; Humans ; Isotopes ; Muscle ; Paediatrics and Reproductive Medicine ; Pediatric ; Pediatrics ; Photon ; Preschool ; Public Health and Health Services ; Skeletal
  • É parte de: Pediatric Research, 2023-09, Vol.94 (3), p.1195-1202
  • Notas: 1195 - 1202
    Pediatric Research, vol 94, iss 3
  • Descrição: BackgroundGiven limited experience in applying the creatine-(methyl-D3) (D3Cr) dilution method to measure skeletal muscle mass (SMM) in young children, the feasibility of deployment in a fielding setting and performance of the method was assessed in a cohort of 4-year-old children in Dhaka, Bangladesh.MethodsFollowing D3Cr oral dose (10 mg) administration, single fasting urine samples were collected at 2-4 days (n = 100). Twenty-four-hour post-dose collections and serial spot urine samples on days 2, 3 and 4 were obtained in a subset of participants (n = 10). Urinary creatine, creatinine, D3Cr and D3-creatinine enrichment were analyzed by liquid chromatography-tandem mass spectrometry. Appendicular lean mass (ALM) was measured by dual-energy x-ray absorptiometry and grip strength was measured by a hand-held dynamometer.ResultsSMM was measured successfully in 91% of participants, and there were no adverse events. Mean ± SD SMM was greater than ALM (4.5 ± 0.4 and 3.2 ± 0.6 kg, respectively). Precision of SMM was low (intraclass correlation = 0.20; 95% CI: 0.02, 0.75; n = 10). Grip strength was not associated with SMM in multivariable analysis (0.004 kg per 100 g of SMM; 95% CI: -0.031, 0.038; n = 91).ConclusionsThe D3Cr dilution method was feasible in a community setting. However, high within-child variability in SMM estimates suggests the need for further optimization of this approach.ImpactThe D3-creatine (D3Cr) stable isotope dilution method was considered a feasible method for the estimation of skeletal muscle mass (SMM) in young children in a community setting and was well accepted among participants. SMM was weakly associated with both dual-energy x-ray absorptiometry-derived values of appendicular lean mass and grip strength. High within-child variability in estimated values of SMM suggests that further optimization of the D3Cr stable isotope dilution method is required prior to implementation in community research settings.
  • Editor: eScholarship, University of California
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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