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LED therapy plus idebenone treatment targeting calcium and mitochondrial signaling pathways in dystrophic muscle cells

da Silva, Heloina Nathalliê Mariano ; Mizobuti, Daniela Sayuri ; Pereira, Valéria Andrade ; da Rocha, Guilherme Luiz ; da Cruz, Marcos Vinícius ; de Oliveira, André Gustavo ; Silveira, Leonardo Reis ; Minatel, Elaine

Cell stress & chaperones, 2023-11, Vol.28 (6), p.773-785 [Periódico revisado por pares]

Dordrecht: Springer Science + Business Media

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Título:
LED therapy plus idebenone treatment targeting calcium and mitochondrial signaling pathways in dystrophic muscle cells
Autor: da Silva, Heloina Nathalliê Mariano ; Mizobuti, Daniela Sayuri ; Pereira, Valéria Andrade ; da Rocha, Guilherme Luiz ; da Cruz, Marcos Vinícius ; de Oliveira, André Gustavo ; Silveira, Leonardo Reis ; Minatel, Elaine
Assuntos: Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Ca2+-transporting ATPase ; Calcium ; Calcium (intracellular) ; Calcium (mitochondrial) ; Calcium - metabolism ; Calcium signalling ; Calpain ; Calsequestrin ; Cancer Research ; Cell Biology ; Cytotoxicity ; Disease Models, Animal ; Duchenne's muscular dystrophy ; Dystrophy ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Immunology ; Intracellular ; Intracellular signalling ; Light effects ; Light emitting diodes ; Mice ; Mice, Inbred C57BL ; Mice, Inbred mdx ; Mitochondria ; Muscle Cells - metabolism ; Muscle, Skeletal - metabolism ; Muscles ; Muscular dystrophy ; Neurosciences ; Original ; ORIGINAL ARTICLE ; Oxidative stress ; Phenotypes ; Reduction ; Sarcolipin ; Signal Transduction
É parte de: Cell stress & chaperones, 2023-11, Vol.28 (6), p.773-785
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Descrição: Intracellular calcium dysregulation, oxidative stress, and mitochondrial dysfunction are some of the main pathway contributors towards disease progression in Duchenne muscular dystrophy (DMD). This study is aimed at investigating the effects of light emitting diode therapy (LEDT) and idebenone antioxidant treatment, applied alone or together in dystrophic primary muscle cells from mdx mice, the experimental model of DMD. Mdx primary muscle cells were submitted to LEDT and idebenone treatment and evaluated for cytotoxic effects and calcium and mitochondrial signaling pathways. LEDT and idebenone treatment showed no cytotoxic effects on the dystrophic muscle cells. Regarding the calcium pathways, after LEDT and idebenone treatment, a significant reduction in intracellular calcium content, calpain-1, calsequestrin, and sarcolipin levels, was observed. In addition, a significant reduction in oxidative stress level markers, such as H₂O₂, and 4-HNE levels, was observed. Regarding mitochondrial signaling pathways, a significant increase in oxidative capacity (by OCR and OXPHOS levels) was observed. In addition, the PGC-1α, SIRT-1, and PPARδ levels were significantly higher in the LEDT plus idebenone treated-dystrophic muscle cells. Together, the findings suggest that LEDT and idebenone treatment, alone or in conjunction, can modulate the calcium and mitochondrial signaling pathways, such as SLN, SERCA 1, and PGC-1α, contributing towards the improvement of the dystrophic phenotype in mdx muscle cells. In addition, data from the LEDT plus idebenone treatment showed slightly better results than those of each separate treatment in terms of SLN, OXPHOS, and SIRT-1.
Editor: Dordrecht: Springer Science + Business Media
Idioma: Inglês

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LED therapy plus idebenone treatment targeting calcium and mitochondrial signaling pathways in dystrophic muscle cells

da Silva, Heloina Nathalliê Mariano ; Mizobuti, Daniela Sayuri ; Pereira, Valéria Andrade ; da Rocha, Guilherme Luiz ; da Cruz, Marcos Vinícius ; de Oliveira, André Gustavo ; Silveira, Leonardo Reis ; Minatel, Elaine

Dordrecht: Springer Science + Business Media

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