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MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato

Zhu, Lingcheng ; Li, Baiyun ; Wu, Limin ; Li, Huixia ; Wang, Zhengyang ; Wei, Xiaoyu ; Ma, Baiquan ; Zhang, Yanfeng ; Ma, Fengwang ; Ruan, Yong-Ling ; Li, Mingjun

Proceedings of the National Academy of Sciences - PNAS, 2021-01, Vol.118 (1), p.1 [Periódico revisado por pares]

United States: National Academy of Sciences

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Título:
MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato
Autor: Zhu, Lingcheng ; Li, Baiyun ; Wu, Limin ; Li, Huixia ; Wang, Zhengyang ; Wei, Xiaoyu ; Ma, Baiquan ; Zhang, Yanfeng ; Ma, Fengwang ; Ruan, Yong-Ling ; Li, Mingjun
Assuntos: Accumulation ; Apples ; Biological Sciences ; Cytosol ; Cytosol - metabolism ; Efflux ; Fructose - metabolism ; Fruit - metabolism ; Fruits ; Gene expression ; Gene Expression Regulation, Plant - genetics ; Gene Knockout Techniques ; Gene sequencing ; Gene Silencing ; Glucose ; Glucose - metabolism ; Homeostasis ; Hydrogen ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - metabolism ; Malus - genetics ; Malus - metabolism ; Monosaccharide Transport Proteins - genetics ; Monosaccharide Transport Proteins - metabolism ; Phylogeny ; Plant cells ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Promoter Regions, Genetic ; Regulatory mechanisms (biology) ; Ribonucleic acid ; RNA ; RNA-Seq ; Sucrose ; Sucrose - metabolism ; Sugar ; Sugars ; Tomatoes ; Tonoplasts ; Up-Regulation ; Vacuoles ; Vacuoles - metabolism
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2021-01, Vol.118 (1), p.1
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by Natasha V. Raikhel, Center for Plant Cell Biology, Riverside, CA, and approved November 17, 2020 (received for review November 11, 2020)
Author contributions: L.Z., F.M., Y.-L.R., and M.L. designed research; L.Z., B.L., H.L., Z.W., and X.W. performed research; L.Z., L.W., B.M., Y.Z., Y.-L.R., and M.L. analyzed data; L.Z., Y.-L.R., and M.L. wrote the paper; and F.M., Y.-L.R., and M.L. supervised research.
Descrição: Sugar transport across tonoplasts is essential for maintaining cellular sugar homeostasis and metabolic balance in plant cells. It remains unclear, however, how this process is regulated among different classes of sugar transporters. Here, we identified a tonoplast H /glucose symporter, MdERDL6-1, from apples, which was highly expressed in fruits and exhibited expression patterns similar to those of the tonoplast H /sugar antiporters MdTST1 and MdTST2. Overexpression of unexpectedly increased not only glucose (Glc) concentration but also that of fructose (Fru) and sucrose (Suc) in transgenic apple and tomato leaves and fruits. RNA sequencing (RNA-seq) and expression analyses showed an up-regulation of and in the transgenic apple and tomato lines overexpressing Further studies established that the increased sugar concentration in the transgenic lines correlated with up-regulation of and expression. Suppression or knockout of and in the overexpressed tomato background reduced or abolished the positive effect of on sugar accumulation, respectively. The findings demonstrate a regulation of and by , in which Glc exported by MdERDL6-1 from vacuole up-regulates and to import sugars from cytosol to vacuole for accumulation to high concentrations. The results provide insight into the regulatory mechanism of sugar accumulation in vacuoles mediated by the coordinated action of two classes of tonoplast sugar transporters.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato

Zhu, Lingcheng ; Li, Baiyun ; Wu, Limin ; Li, Huixia ; Wang, Zhengyang ; Wei, Xiaoyu ; Ma, Baiquan ; Zhang, Yanfeng ; Ma, Fengwang ; Ruan, Yong-Ling ; Li, Mingjun

United States: National Academy of Sciences

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