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Transcriptome analysis in venom gland of the predatory giant ant Dinoponera quadriceps: insights into the polypeptide toxin arsenal of hymenopterans

Torres, Alba F C ; Huang, Chen ; Chong, Cheong-Meng ; Leung, Siu Wai ; Prieto-da-Silva, Alvaro R B ; Havt, Alexandre ; Quinet, Yves P ; Martins, Alice M C ; Lee, Simon M Y ; Rádis-Baptista, Gandhi Ho, Paulo Lee

PloS one, 2014-01, Vol.9 (1), p.e87556 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Transcriptome analysis in venom gland of the predatory giant ant Dinoponera quadriceps: insights into the polypeptide toxin arsenal of hymenopterans
Autor: Torres, Alba F C ; Huang, Chen ; Chong, Cheong-Meng ; Leung, Siu Wai ; Prieto-da-Silva, Alvaro R B ; Havt, Alexandre ; Quinet, Yves P ; Martins, Alice M C ; Lee, Simon M Y ; Rádis-Baptista, Gandhi
Ho, Paulo Lee
Assuntos: Allergens ; Analgesics ; Analysis ; Animals ; Ant Venoms - biosynthesis ; Ant Venoms - genetics ; Ants - genetics ; Ants - metabolism ; Apis mellifera ; Biology ; Bites and stings ; Camponotus floridanus ; Chemistry ; Computer programs ; Cysteine ; Dinoponera ; Dinoponera quadriceps ; DNA sequencing ; Ecology ; Esterases ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation - physiology ; Gene sequencing ; Genomes ; Genomic libraries ; Genomics ; Gram-positive bacteria ; Harpegnathos saltator ; Humans ; Hymenoptera ; Insect Proteins - biosynthesis ; Insect Proteins - genetics ; Insects ; Laboratories ; Medicine ; Myrmecia ; Myrmecia pilosula ; Nucleotide sequencing ; Pain ; Peptides ; Pharmacy ; Pogonomyrmex barbatus ; Ponerinae ; Predators ; Prey ; Proteins ; Quadriceps muscle ; R&D ; Research & development ; Stings ; Stings (Injuries) ; Toxins ; Transcriptome - physiology ; Vegetation ; Venom ; Venom gland ; Venoms
É parte de: PloS one, 2014-01, Vol.9 (1), p.e87556
Notas: Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: AMCM ARBPS SMYL GR-B. Performed the experiments: AFCT CH AH C-MC YPQ. Analyzed the data: AFCT CH C-MC ARBPS SWL SMYL GR-B. Contributed reagents/materials/analysis tools: AMCM ARBPS SMYL GR-B. Wrote the paper: AFCT CH ARBPS SMYL GR-B. Field trip missions and rearing ants: AFCT YPQ.
Descrição: Dinoponera quadriceps is a predatory giant ant that inhabits the Neotropical region and subdues its prey (insects) with stings that deliver a toxic cocktail of molecules. Human accidents occasionally occur and cause local pain and systemic symptoms. A comprehensive study of the D. quadriceps venom gland transcriptome is required to advance our knowledge about the toxin repertoire of the giant ant venom and to understand the physiopathological basis of Hymenoptera envenomation. We conducted a transcriptome analysis of a cDNA library from the D. quadriceps venom gland with Sanger sequencing in combination with whole-transcriptome shotgun deep sequencing. From the cDNA library, a total of 420 independent clones were analyzed. Although the proportion of dinoponeratoxin isoform precursors was high, the first giant ant venom inhibitor cysteine-knot (ICK) toxin was found. The deep next generation sequencing yielded a total of 2,514,767 raw reads that were assembled into 18,546 contigs. A BLAST search of the assembled contigs against non-redundant and Swiss-Prot databases showed that 6,463 contigs corresponded to BLASTx hits and indicated an interesting diversity of transcripts related to venom gene expression. The majority of these venom-related sequences code for a major polypeptide core, which comprises venom allergens, lethal-like proteins and esterases, and a minor peptide framework composed of inter-specific structurally conserved cysteine-rich toxins. Both the cDNA library and deep sequencing yielded large proportions of contigs that showed no similarities with known sequences. To our knowledge, this is the first report of the venom gland transcriptome of the New World giant ant D. quadriceps. The glandular venom system was dissected, and the toxin arsenal was revealed; this process brought to light novel sequences that included an ICK-folded toxins, allergen proteins, esterases (phospholipases and carboxylesterases), and lethal-like toxins. These findings contribute to the understanding of the ecology, behavior and venomics of hymenopterans.
Editor: United States: Public Library of Science
Idioma: Inglês

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Transcriptome analysis in venom gland of the predatory giant ant Dinoponera quadriceps: insights into the polypeptide toxin arsenal of hymenopterans

Torres, Alba F C ; Huang, Chen ; Chong, Cheong-Meng ; Leung, Siu Wai ; Prieto-da-Silva, Alvaro R B ; Havt, Alexandre ; Quinet, Yves P ; Martins, Alice M C ; Lee, Simon M Y ; Rádis-Baptista, Gandhi Ho, Paulo Lee

United States: Public Library of Science

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