skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Modulating the Properties of Metal-Sensing Whole-Cell Bioreporters by Interfering with Escherichia coli Metal Homeostasis

Yoon, Youngdae ; Kang, Yerin ; Lee, Woonwoo ; Oh, Ki-Cheol ; Jang, Geupil ; Kim, Bong-Gyu

Journal of microbiology and biotechnology, 2018-02, Vol.28 (2), p.323-329 [Periódico revisado por pares]

한국미생물생명공학회

Sem texto completo

Citações Citado por
  • Título:
    Modulating the Properties of Metal-Sensing Whole-Cell Bioreporters by Interfering with Escherichia coli Metal Homeostasis
  • Autor: Yoon, Youngdae ; Kang, Yerin ; Lee, Woonwoo ; Oh, Ki-Cheol ; Jang, Geupil ; Kim, Bong-Gyu
  • Assuntos: Arsenic ; cadmium ; Escherichia coli ; metal homeostasis ; whole-cell bioreporter ; zntoperon
  • É parte de: Journal of microbiology and biotechnology, 2018-02, Vol.28 (2), p.323-329
  • Notas: The Korean Society for Applied Microbiology
    KISTI1.1003/JNL.JAKO201810038011810
  • Descrição: In Escherichia coli, the transcription of genes related to metal homeostasis is activated by the presence of target metals. The promoter regions of those genes can be fused with reporter genes to generate whole-cell bioreporters (WCBs); these organisms sense the presence of target metals through reporter gene expression. However, the limited number of available promoters for sensing domains restricts the number of WCB targets. In this study, we have demonstrated an alternative method to generate novel WCBs, based on the notion that since the sensing mechanisms of WCBs are related to metal transportation systems, their properties can be modulated by disrupting metal homeostasis. Mutant E. coli strains were generated by deleting the znt-operon genes zntA, which encodes a zinc-export protein, and zntR, which encodes a znt-operon regulatory protein, to investigate the effects on the metal-sensing properties of WCBs. Deletion of zntA increased the sensitivity but abolished the selectivity of cadmiumsensing WCBs, whereas arsenic-sensing WCBs gained sensitivity toward cadmium. When zntR was deleted, cadmium-sensing WCBs lost the ability to detect cadmium, and this was recovered by introducing exogenous zntR. In addition, the metal-binding site of ZntR was genetically engineered to modulate metal selectivity. This study provides a valuable platform for the development of novel E. coli-based WCBs.
  • Editor: 한국미생물생명공학회
  • Idioma: Coreano
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.