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Glutamine supplementation prevents exercise-induced neutrophil apoptosis and reduces p38 MAPK and JNK phosphorylation and p53 and caspase 3 expression

Lagranha, Claudia J. ; Hirabara, Sandro M. ; Curi, Rui ; Pithon-Curi, Tania C.

Cell biochemistry and function, 2007-09, Vol.25 (5), p.563-569 [Periódico revisado por pares]

Chichester, UK: John Wiley & Sons, Ltd

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Glutamine supplementation prevents exercise-induced neutrophil apoptosis and reduces p38 MAPK and JNK phosphorylation and p53 and caspase 3 expression
Autor: Lagranha, Claudia J. ; Hirabara, Sandro M. ; Curi, Rui ; Pithon-Curi, Tania C.
Assuntos: Animals ; apoptosis ; Apoptosis - drug effects ; caspase 3 ; Caspase 3 - metabolism ; Dietary Supplements ; glutamine ; Glutamine - administration & dosage ; Glutamine - pharmacology ; JNK Mitogen-Activated Protein Kinases - metabolism ; Male ; neutrophils ; Neutrophils - cytology ; Neutrophils - drug effects ; p38 MAPK/JNK pathway ; p38 Mitogen-Activated Protein Kinases - metabolism ; p53 ; Phosphorylation - drug effects ; Physical Conditioning, Animal - physiology ; Rats ; Rats, Wistar ; single session of exercise ; Tumor Suppressor Protein p53 - metabolism
É parte de: Cell biochemistry and function, 2007-09, Vol.25 (5), p.563-569
Notas: FAPESP
CNPq
Claudia J. Lagranha and Sandro M. Hirabara are equally contributed to this work.
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Descrição: We have previously shown that a single session of exercise induces DNA fragmentation, mitochondrial membrane depolarization, increases expression of pro‐apoptotic genes (bax and bcl‐xS) and decreases expression of anti‐apoptotic genes (bcl‐xL) in rat neutrophils. Glutamine supplementation had a protective effect in the apoptosis induced by a single session of exercise. The mechanism involved in the effect of single session of exercise to induce apoptosis was investigated by measuring expression of p53 and caspase 3 and phosphorylation of p38 mitogen‐activated protein kinases (MAPK) and cJun NH2‐terminal kinase (JNK) in neutrophils from rats supplemented or not with glutamine. Exercise was carried out on a treadmill for 1 h and the rats were killed by decapitation. Neutrophils were obtained by intraperitoneal (i.p.) lavage with PBS, 4 h after injection of oyster glycogen solution. Glutamine supplementation (1g per Kg b.w.) was given by gavage 1 h before the exercise session. Gene expression and protein phosphorylation were then analyzed by reverse transcriptase chain reaction (RT‐PCR) and Western blotting, respectively. A single session of exercise increased p38 MAPK and JNK phosphorylation and p53 and caspase 3 expression. Glutamine supplementation partially prevented the increase in p38 MAPK and JNK phosphorylation and p53 expression, and fully abolished the increase in caspase 3 expression. Thus, neutrophil apoptosis induced by a single session of exercise is accompanied by increased p53 and caspase 3 expression and p38 MAPK and JNK phosphorylation. Glutamine supplementation prevents these effects of exercise and reduces apoptosis. Copyright © 2007 John Wiley & Sons, Ltd.
Editor: Chichester, UK: John Wiley & Sons, Ltd
Idioma: Inglês

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Glutamine supplementation prevents exercise-induced neutrophil apoptosis and reduces p38 MAPK and JNK phosphorylation and p53 and caspase 3 expression

Lagranha, Claudia J. ; Hirabara, Sandro M. ; Curi, Rui ; Pithon-Curi, Tania C.

Chichester, UK: John Wiley & Sons, Ltd

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