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Structural Characterisation of Human Recombinant Glycohormones Follitropin, Lutropin and Choriogonadotropin Expressed in Chinese Hamster Ovary Cells

Amoresano, Angela ; Siciliano, Rosa ; Orrù, Stefania ; Napoleoni, Roberta ; Altarocca, Valter ; Luca, Eva ; Sirna, Antonino ; Pucci, Piero

European journal of biochemistry, 1996-12, Vol.242 (3), p.608-618 [Periódico revisado por pares]

Oxford, UK: Blackwell Science Ltd

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Título:
Structural Characterisation of Human Recombinant Glycohormones Follitropin, Lutropin and Choriogonadotropin Expressed in Chinese Hamster Ovary Cells
Autor: Amoresano, Angela ; Siciliano, Rosa ; Orrù, Stefania ; Napoleoni, Roberta ; Altarocca, Valter ; Luca, Eva ; Sirna, Antonino ; Pucci, Piero
Assuntos: Amino Acid Sequence ; Animals ; CHO Cells ; Chorionic Gonadotropin - chemistry ; Cricetinae ; Follicle Stimulating Hormone - chemistry ; Glycoproteins - chemistry ; gonadotropin ; Humans ; Luteinizing Hormone - chemistry ; Mass Spectrometry ; Molecular Sequence Data ; oligosaccharide ; Oligosaccharides - chemistry ; recombinant glycoprotein ; Recombinant Proteins - chemistry ; structural characterisation
É parte de: European journal of biochemistry, 1996-12, Vol.242 (3), p.608-618
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The α and β chains from human recombinant gonadotropins follitropin, lutropin and choriogonadotropin expressed in CHO cells have been structurally characterised both at the protein and at the carbohydrate level by using advanced mass spectrometric procedures. The three α chains share the same amino acid sequence while they display different glycosylation patterns. The oligosaccharide structures detected belong to the complex‐type glycans with different degree of sialylation. Partial proteolytic processing occurred at the N‐terminus of the follitropin β chain and at the C‐terminus of the lutropin β chain. The N‐linked glycans from the three β chains were found to contain fucosylated and sialylated diantennary and triantennary complex‐type structures. The follitropin β chain showed the presence of N‐acetyllactos‐amine repeats on the antennae. The overall structure of the recombinant glycohormones corresponds to their natural counterparts with the exception that sulphated terminal glycosylation is missing.
Editor: Oxford, UK: Blackwell Science Ltd
Idioma: Inglês

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Structural Characterisation of Human Recombinant Glycohormones Follitropin, Lutropin and Choriogonadotropin Expressed in Chinese Hamster Ovary Cells

Amoresano, Angela ; Siciliano, Rosa ; Orrù, Stefania ; Napoleoni, Roberta ; Altarocca, Valter ; Luca, Eva ; Sirna, Antonino ; Pucci, Piero

Oxford, UK: Blackwell Science Ltd

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