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The chaperone-like protein 14-3-3η interacts with human α-synuclein aggregation intermediates rerouting the amyloidogenic pathway and reducing α-synuclein cellular toxicity

Plotegher, Nicoletta ; Kumar, Dhruv ; Tessari, Isabella ; Brucale, Marco ; Munari, Francesca ; Tosatto, Laura ; Belluzzi, Elisa ; Greggio, Elisa ; Bisaglia, Marco ; Capaldi, Stefano ; Aioanei, Daniel ; Mammi, Stefano ; Monaco, Hugo L ; Samo, Bruno ; Bubacco, Luigi

Human molecular genetics, 2014-11, Vol.23 (21), p.5615-5629 [Periódico revisado por pares]

England

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Título:
The chaperone-like protein 14-3-3η interacts with human α-synuclein aggregation intermediates rerouting the amyloidogenic pathway and reducing α-synuclein cellular toxicity
Autor: Plotegher, Nicoletta ; Kumar, Dhruv ; Tessari, Isabella ; Brucale, Marco ; Munari, Francesca ; Tosatto, Laura ; Belluzzi, Elisa ; Greggio, Elisa ; Bisaglia, Marco ; Capaldi, Stefano ; Aioanei, Daniel ; Mammi, Stefano ; Monaco, Hugo L ; Samo, Bruno ; Bubacco, Luigi
Assuntos: 14-3-3 Proteins - chemistry ; 14-3-3 Proteins - genetics ; 14-3-3 Proteins - metabolism ; alpha-Synuclein - chemistry ; alpha-Synuclein - genetics ; alpha-Synuclein - metabolism ; Amyloidosis - metabolism ; Humans ; Kinetics ; Protein Aggregation, Pathological ; Protein Binding ; Protein Isoforms ; Signal Transduction
É parte de: Human molecular genetics, 2014-11, Vol.23 (21), p.5615-5629
Descrição: Familial and idiopathic Parkinson's disease (PD) is associated with the abnormal neuronal accumulation of α-synuclein (aS) leading to β-sheet-rich aggregates called Lewy Bodies (LBs). Moreover, single point mutation in aS gene and gene multiplication lead to autosomal dominant forms of PD. A connection between PD and the 14-3-3 chaperone-like proteins was recently proposed, based on the fact that some of the 14-3-3 isoforms can interact with genetic PD-associated proteins such as parkin, LRRK2 and aS and were found as components of LBs in human PD. In particular, a direct interaction between 14-3-3η and aS was reported when probed by co-immunoprecipitation from cell models, from parkinsonian brains and by surface plasmon resonance in vitro. However, the mechanisms through which 14-3-3η and aS interact in PD brains remain unclear. Herein, we show that while 14-3-3η is unable to bind monomeric aS, it interacts with aS oligomers which occur during the early stages of aS aggregation. This interaction diverts the aggregation process even when 14-3-3η is present in sub-stoichiometric amounts relative to aS. When aS level is overwhelmingly higher than that of 14-3-3η, the fibrillation process becomes a sequestration mechanism for 14-3-3η, undermining all processes governed by this protein. Using a panel of complementary techniques, we single out the stage of aggregation at which the aS/14-3-3η interaction occurs, characterize the products of the resulting processes, and show how the processes elucidated in vitro are relevant in cell models. Our findings constitute a first step in elucidating the molecular mechanism of aS/14-3-3η interaction and in understanding the critical aggregation step at which 14-3-3η has the potential to rescue aS-induced cellular toxicity.
Editor: England
Idioma: Inglês

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The chaperone-like protein 14-3-3η interacts with human α-synuclein aggregation intermediates rerouting the amyloidogenic pathway and reducing α-synuclein cellular toxicity

Plotegher, Nicoletta ; Kumar, Dhruv ; Tessari, Isabella ; Brucale, Marco ; Munari, Francesca ; Tosatto, Laura ; Belluzzi, Elisa ; Greggio, Elisa ; Bisaglia, Marco ; Capaldi, Stefano ; Aioanei, Daniel ; Mammi, Stefano ; Monaco, Hugo L ; Samo, Bruno ; Bubacco, Luigi

England

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