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Carboxymethyl cellulase production optimization from Glutamicibacter arilaitensis strain ALA4 and its application in lignocellulosic waste biomass saccharification

Aarti, Chirom ; Khusro, Ameer ; Agastian, Paul

Preparative biochemistry & biotechnology, 2018-10, Vol.48 (9), p.853-866 [Periódico revisado por pares]

England: Taylor & Francis

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Título:
Carboxymethyl cellulase production optimization from Glutamicibacter arilaitensis strain ALA4 and its application in lignocellulosic waste biomass saccharification
Autor: Aarti, Chirom ; Khusro, Ameer ; Agastian, Paul
Assuntos: Animals ; Biomass ; Carboxymethyl cellulase ; Cell Culture Techniques - methods ; Cellulase ; Cellulase - biosynthesis ; Cellulase - chemistry ; Dung ; Factorial design ; Feces - microbiology ; Fermentation ; Glutamicibacter arilaitensis ; Glycosylation ; goat dung ; Goat manure ; Goats ; Hydrogen-Ion Concentration ; Lignin - chemistry ; Lignin - metabolism ; Lignocellulose ; lignocellulosic wastes ; Metal ions ; Micrococcaceae - enzymology ; Micrococcaceae - metabolism ; Moisture ; Optimization ; Organic solvents ; Peptone ; pH effects ; Pollutants ; Polynomials ; Protein Stability ; Saccharification ; Straw ; Sugars ; Surfactants ; Temperature
É parte de: Preparative biochemistry & biotechnology, 2018-10, Vol.48 (9), p.853-866
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: In this context, carboxymethyl cellulase (CMCase) production from Glutamicibacter arilaitensis strain ALA4 was initially optimized by one factor at a time (OFAT) method using goat dung as proficient feedstock. Two-level full factorial design (2 5 factorial matrix) using first-order polynomial model revealed the significant (p < 0.05) influence of pH, moisture, and peptone on CMCase activity. Central composite design at N = 20 was further taken into account using a second-order polynomial equation, and thereby liberated maximum CMCase activity of 4925.56 ± 31.61 U/g in the goat dung medium of pH 8.0 and 100% moisture containing 1% (w/w) peptone, which was approximately two fold increment with respect to OFAT method. Furthermore, the partially purified CMCase exhibited stability not only at high pH and temperature but also in the presence of varied metal ions, organic solvents, surfactants, and inhibitors with pronounced residual activities. The enzymatic hydrolysis using partially purified CMCase depicted the maximum liberation of fermentable sugars from alkali pretreated lignocellulosic wastes biomass in the order of paddy straw (13.8 ± 0.15 mg/g) > pomegranate peel (9.1 ± 0.18 mg/g) > sweet lime peel (8.37 ± 0.16 mg/g), with saccharification efficiency of 62.1 ± 0.8, 40.95 ± 0.4, and 37.66 ± 0.4%, respectively after 72 hr of treatment.
Editor: England: Taylor & Francis
Idioma: Inglês

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Carboxymethyl cellulase production optimization from Glutamicibacter arilaitensis strain ALA4 and its application in lignocellulosic waste biomass saccharification

Aarti, Chirom ; Khusro, Ameer ; Agastian, Paul

England: Taylor & Francis

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