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Evaluation of Sandwich ELISA Galactomannan Test in Samples of Positive LA Test and Positive Aspergillus Antibody

MAESAKI, Shigefumi ; KAWAMURA, Sumio ; HASHIGUCHI, Kohji ; HOSSAIN, Mohammad Ashraf ; SASAKI, Eisuke ; MIYAZAKI, Yoshitsugu ; TOMONO, Kazunori ; TASHIRO, Takayoshi ; KOHNO, Shigeru

Internal Medicine, 1999, Vol.38(12), pp.948-950 [Periódico revisado por pares]

Tokyo: The Japanese Society of Internal Medicine

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Título:
Evaluation of Sandwich ELISA Galactomannan Test in Samples of Positive LA Test and Positive Aspergillus Antibody
Autor: MAESAKI, Shigefumi ; KAWAMURA, Sumio ; HASHIGUCHI, Kohji ; HOSSAIN, Mohammad Ashraf ; SASAKI, Eisuke ; MIYAZAKI, Yoshitsugu ; TOMONO, Kazunori ; TASHIRO, Takayoshi ; KOHNO, Shigeru
Assuntos: anti-Aspergillus antibody ; Antibodies, Fungal - blood ; Antigens, Fungal - blood ; aspergilloma ; aspergillosis ; Aspergillosis - blood ; Aspergillosis - diagnosis ; Aspergillus - immunology ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay - methods ; Evaluation Studies as Topic ; Humans ; invasive aspergillosis ; Investigative techniques, diagnostic techniques (general aspects) ; Latex Fixation Tests ; Mannans - immunology ; Medical sciences ; Miscellaneous. Technology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Predictive Value of Tests ; serodiagnosis
É parte de: Internal Medicine, 1999, Vol.38(12), pp.948-950
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: Objective The detection of circulating Aspergillus galactomannan antigen is a useful tool for serodiagnosis of aspergillosis. However, the latex agglutination test for the detection of galactomannan is not completely reliable due to it's low sensitivity. The sandwich ELISA was developed to achieve high sensitivity. Materials The sandwich immunocapture ELISA was evaluated by testing 56 sero-positive and 56 sero-negative samples of circulating galactomannan detected by LA test retrospectively. Results Sixty of the samples were positive for galactomannan as measured by sandwich ELISA. Fifteen samples out of 56 samples negative by LA test were positive by ELISA and 4 samples out of 56 samples positive by LA test were negative by ELISA. Among 47 serum samples positive for anti-Aspergillus antibody, 14 samples were positive by ELISA. Conclusion In conclusion, galactomannan may be detected in more samples of by the new sandwich ELISA than by LA test. (Internal Medicine 38: 948-950, 1999)
Editor: Tokyo: The Japanese Society of Internal Medicine
Idioma: Inglês

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Evaluation of Sandwich ELISA Galactomannan Test in Samples of Positive LA Test and Positive Aspergillus Antibody

MAESAKI, Shigefumi ; KAWAMURA, Sumio ; HASHIGUCHI, Kohji ; HOSSAIN, Mohammad Ashraf ; SASAKI, Eisuke ; MIYAZAKI, Yoshitsugu ; TOMONO, Kazunori ; TASHIRO, Takayoshi ; KOHNO, Shigeru

Tokyo: The Japanese Society of Internal Medicine

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