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Comparison of Celsior and Perfadex lung preservation solutions in rat lungs subjected to 6 and 12 hours of ischemia using an ex-vivo lung perfusion system

Menezes, Arteiro Queiroz; Pêgo-Fernandes, Paulo Manuel; Cardoso, Paulo Francisco Guerreiro; Braga, Karina Andrighetti De Oliveira; Nepomuceno, Natalia Aparecida; Pazetti, Rogerio; Correia, Aristides Tadeu; Canzian, Mauro; Santim, Jacqueline Klarosk; Jatene, Fabio Biscegli

Clinics; v. 67 n. 11 (2012); 1309-1314

Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo 2012-11-01

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  • Título:
    Comparison of Celsior and Perfadex lung preservation solutions in rat lungs subjected to 6 and 12 hours of ischemia using an ex-vivo lung perfusion system
  • Autor: Menezes, Arteiro Queiroz; Pêgo-Fernandes, Paulo Manuel; Cardoso, Paulo Francisco Guerreiro; Braga, Karina Andrighetti De Oliveira; Nepomuceno, Natalia Aparecida; Pazetti, Rogerio; Correia, Aristides Tadeu; Canzian, Mauro; Santim, Jacqueline Klarosk; Jatene, Fabio Biscegli
  • Assuntos: Lung Transplantation; Organ Preservation Solutions; Animal Experiment
  • É parte de: Clinics; v. 67 n. 11 (2012); 1309-1314
  • Descrição: OBJECTIVE: This study evaluated the performance of lungs that were preserved with different solutions (Celsior, Perfadex or saline) in an ex vivo rat lung perfusion system. METHODS: Sixty Wistar rats were anesthetized, anticoagulated and randomized into three groups (n = 20). The rats were subjected to antegrade perfusion via the pulmonary artery with Perfadex, Celsior, or saline, followed by 6 or 12 hours of ischemia (4ºC, n = 10 in each group). Respiratory mechanics, gas exchange and hemodynamics were measured at 10-minute intervals during the reperfusion of heart-lung blocks in an ex vivo system (IL2-Isolated Perfused Rat or Guinea Pig Lung System, Harvard Apparatus, Holliston, Massachusetts, USA; Hugo Sachs Elektronik, Germany) for 60 minutes. The lungs were prepared for histopathology and evaluated for edema following reperfusion. Group comparisons were performed using ANOVA and the Kruskal-Wallis test with a 5% level of significance. RESULTS: Gas exchange was not significantly different between lungs perfused with either Perfadex or Celsior at the same ischemic times, but it was very low in lungs that were preserved with saline. Airway resistance was greater in the lungs that were preserved for 12 hours. Celsior lungs that were preserved for 6 and 12 hours exhibited lower airway resistance (p = 0.01) compared to Perfadex lungs. Pulmonary artery pressure was not different between the groups, and no significant differences in histopathology and apoptosis were observed between the groups. CONCLUSIONS: Lungs that were preserved with Celsior or Perfadex exhibited similar gas exchange and histopathological findings. Airway resistance was slightly lower in the Celsior-preserved lungs compared with the Perfadex-preserved lungs.
  • Títulos relacionados: https://www.revistas.usp.br/clinics/article/view/47955/51695
  • Editor: Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
  • Data de criação/publicação: 2012-11-01
  • Formato: Adobe PDF
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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