Idioma:

Development and application of an assay for uranyl complexation by fungal metabolites, including siderophores

Renshaw, J.C ; Halliday, V ; Robson, G.D ; Trinci, A.P.J ; Wiebe, M.G ; Livens, F.R ; Collison, D ; Taylor, R.J

Applied and Environmental Microbiology, 2003-06, Vol.69 (6), p.3600-3606 [Periódico revisado por pares]

Washington, DC: American Society for Microbiology

Texto completo disponível

Citações Citado por

Enviar para

Título:
Development and application of an assay for uranyl complexation by fungal metabolites, including siderophores
Autor: Renshaw, J.C ; Halliday, V ; Robson, G.D ; Trinci, A.P.J ; Wiebe, M.G ; Livens, F.R ; Collison, D ; Taylor, R.J
Assuntos: Actinomycetales - growth & development ; Actinomycetales - metabolism ; Aureobasidium ; Bacteria ; Biological and medical sciences ; bioremediation ; chemical reactions ; Culture Media ; Deferoxamine - metabolism ; Fundamental and applied biological sciences. Psychology ; Fungi ; hydroxamic acids ; Hydroxamic Acids - metabolism ; Hydroxybenzoates - metabolism ; Image Processing, Computer-Assisted ; Indicators and Reagents - metabolism ; metabolites ; Microbacterium flavescens ; Microbiological Techniques ; Microbiology ; Mucor ; Mucor - classification ; Mucor - growth & development ; Mucor - isolation & purification ; Mucor - metabolism ; Mycology ; Penicillium ; Penicillium - classification ; Penicillium - growth & development ; Penicillium - isolation & purification ; Penicillium - metabolism ; Siderophores - metabolism ; soil bacteria ; Soil contamination ; soil fungi ; Soil Microbiology ; soil pollution ; uranium ; Uranium - chemistry ; Uranium - metabolism ; yeasts
É parte de: Applied and Environmental Microbiology, 2003-06, Vol.69 (6), p.3600-3606
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Undefined-1
ObjectType-Feature-3
Descrição: An assay to detect UO2(2+) complexation was developed based on the chrome azurol S (CAS) assay for siderophores (B. Schwyn and J. B. Neilands, Anal. Biochem. 160:47-56, 1987) and was used to investigate the ability of fungal metabolites to complex actinides. In this assay the discoloration of two dyed agars (one containing a CAS-Fe3+ dye and the other containing a CAS-UO2(2+) dye) caused by ligands was quantified. The assay was tested by using the siderophore desferrioxamine B (DFO), and the results showed that there was a regular, reproducible relationship between discoloration and the amount of siderophore added. The ratio of the discoloration on the CAS-UO2(2+) agar to the discoloration on the CAS-Fe3+ agar was independent of the amount of siderophore added. A total of 113 fungi and yeasts were isolated from three soil samples taken from the Peak District National Park. The fungi were screened for the production of UO2(2+) chelators by using the CAS-based assay and were also tested specifically for hydroxamate siderophore production by using the hydroxamate siderophore auxotroph Aureobacterium flavescens JG-9. This organism is highly sensitive to the presence of hydroxamate siderophores. However, the CAS-based assay was found to be less sensitive than the A. flavescens JG-9 assay. No significant difference between the results for each site for the two tests was found. Three isolates were selected for further study and were identified as two Pencillium species and a Mucor species. Our results show that the new assay can be effectively used to screen fungi for the production of UO2(2+) chelating ligands. We suggest that hydroxamate siderophores can be produced by mucoraceous fungi.
Editor: Washington, DC: American Society for Microbiology
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Development and application of an assay for uranyl complexation by fungal metabolites, including siderophores

Renshaw, J.C ; Halliday, V ; Robson, G.D ; Trinci, A.P.J ; Wiebe, M.G ; Livens, F.R ; Collison, D ; Taylor, R.J

Washington, DC: American Society for Microbiology

Buscando em bases de dados remotas. Favor aguardar.