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3D organization of synthetic and scrambled chromosomes

Mercy, Guillaume ; Mozziconacci, Julien ; Scolari, Vittore F. ; Yang, Kun ; Zhao, Guanghou ; Thierry, Agnès ; Luo, Yisha ; Mitchell, Leslie A. ; Shen, Michael ; Shen, Yue ; Walker, Roy ; Zhang, Weimin ; Wu, Yi ; Xie, Ze-xiong ; Luo, Zhouqing ; Cai, Yizhi ; Dai, Junbiao ; Yang, Huanming ; Yuan, Ying-Jin ; Boeke, Jef D. ; Bader, Joel S. ; Muller, Héloïse ; Koszul, Romain

Science (American Association for the Advancement of Science), 2017-03, Vol.355 (6329), p.1050-1050 [Periódico revisado por pares]

United States: American Association for the Advancement of Science

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Título:
3D organization of synthetic and scrambled chromosomes
Autor: Mercy, Guillaume ; Mozziconacci, Julien ; Scolari, Vittore F. ; Yang, Kun ; Zhao, Guanghou ; Thierry, Agnès ; Luo, Yisha ; Mitchell, Leslie A. ; Shen, Michael ; Shen, Yue ; Walker, Roy ; Zhang, Weimin ; Wu, Yi ; Xie, Ze-xiong ; Luo, Zhouqing ; Cai, Yizhi ; Dai, Junbiao ; Yang, Huanming ; Yuan, Ying-Jin ; Boeke, Jef D. ; Bader, Joel S. ; Muller, Héloïse ; Koszul, Romain
Assuntos: Baking yeast ; Bins ; Cassettes ; Cell Nucleus - genetics ; Cell Nucleus - ultrastructure ; Centromere - ultrastructure ; Chromosomes ; Chromosomes, Artificial, Yeast - chemistry ; Chromosomes, Artificial, Yeast - genetics ; Chromosomes, Artificial, Yeast - ultrastructure ; Clustering ; Clusters ; Combinatorial analysis ; Contact ; Deletion ; Deoxyribonucleic acid ; Design ; DNA ; DNA, Ribosomal - genetics ; Engineering ; Evolution ; Fitness ; Gene sequencing ; Genes ; Genome, Fungal ; Genomes ; Inversions ; Life Sciences ; Mathematical analysis ; Metabolism ; Nuclear capture ; Nuclear electric power generation ; Nuclei (cytology) ; Nucleic Acid Conformation ; Organizations ; Repetitive Sequences, Nucleic Acid - genetics ; Representations ; RESEARCH ARTICLE SUMMARY ; Ribonucleic acid ; RNA ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Sequence Deletion ; Studies ; Synthetic Biology ; Telomere - ultrastructure ; Tethering ; Trajectories ; Transfer RNA ; Translocation ; Yeasts
É parte de: Science (American Association for the Advancement of Science), 2017-03, Vol.355 (6329), p.1050-1050
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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These authors contributed equally to this work.
Present address: Institut Curie, UMR3664 Dynamique du Noyau, Paris, France.
Descrição: Although the design of the synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, the deletion of several classes of repeated sequences and the introduction of thousands of designer changes may affect genome organization and potentially alter cellular functions. We report here the Hi-C-determined three-dimensional (3D) conformations of Sc2.0 chromosomes. The absence of repeats leads to a smoother contact pattern and more precisely tractable chromosome conformations, and the large-scale genomic organization is globally unaffected by the presence of synthetic chromosome(s). Two exceptions are synIII, which lacks the silent mating-type cassettes, and synXII, specifically when the ribosomal DNA is moved to another chromosome. We also exploit the contact maps to detect rearrangements induced in SCRaMbLE (synthetic chromosome rearrangement and modification by -mediated evolution) strains.
Editor: United States: American Association for the Advancement of Science
Idioma: Inglês

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3D organization of synthetic and scrambled chromosomes

United States: American Association for the Advancement of Science

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