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5‐S RNA: Investigation of the Different Extent of Phosphorylation at 5′ Terminus

Soave, Carlo ; NUCCA, Roberto ; Sala, Elide ; VIOTTI, Angelo ; Galante, Ennio

European journal of biochemistry, 1973-01, Vol.32 (2), p.392-400 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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  • Título:
    5‐S RNA: Investigation of the Different Extent of Phosphorylation at 5′ Terminus
  • Autor: Soave, Carlo ; NUCCA, Roberto ; Sala, Elide ; VIOTTI, Angelo ; Galante, Ennio
  • É parte de: European journal of biochemistry, 1973-01, Vol.32 (2), p.392-400
  • Notas: A
    Definition.
    unit, the quantity of material contained in 1 ml of a solution which has an absorbance of 1 at 260(271) nm, when measured in a 1‐cm pathlength cell.
    260(271)
  • Descrição: 5‐S RNA from wheat germ, homogeneous by gel filtration and polyacrylamide gel electrophoresis, yields three peaks by hydroxyapatite chromatography. Experiments are presented which firmly establish that the three peaks are constituted by 5‐S RNA molecules ending at their 5′ terminus with mono‐, di‐, or triphosphate groups. The isolation of 5‐S RNA by direct extraction of RNA from wheat embryos homogenized in different buffers and the inability of Escherichia coli alkaline phosphatase to dephosphorylate unmelted 5‐S RNA molecules suggests that the three species are not an artifact of extraction. The peculiar ability of the hydroxyapatite chromatography to fractionate 5‐S RNA molecules on the basis of the extent of their 5′‐terminal phosphorylation was used to investigate the pattern of 5′‐phosphorylation of 5‐S RNA from various organisms. The most striking feature observed is that 5‐S RNAs from prokaryotic organisms contain only 5′‐monophosphate molecules. In contrast, the presence of 5′ mono‐, di‐ and triphosphate molecules was observed in all the eukaryotic organisms investigated. The significance of this finding in relation to the biosynthesis of 5‐S RNA is discussed.
  • Editor: Oxford, UK: Blackwell Publishing Ltd
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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