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Role of Oxidative Stress, Apoptosis. and Intracellular Homeostasis in Primary Cultures of Rat Proximal Tubular Cells Exposed to Cadmium

Wang, L ; Cao, J ; Chen, D ; Liu, X ; Lu, H

Biological trace element research, 1901-01, Vol.127 (1), p.53-68 [Periódico revisado por pares]

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  • Título:
    Role of Oxidative Stress, Apoptosis. and Intracellular Homeostasis in Primary Cultures of Rat Proximal Tubular Cells Exposed to Cadmium
  • Autor: Wang, L ; Cao, J ; Chen, D ; Liu, X ; Lu, H
  • Assuntos: Acetic acid ; Acetylcysteine ; Acidification ; Apoptosis ; Ca super(2+)-transporting ATPase ; Cadmium ; Calcium ; Cell culture ; Cytotoxicity ; Glutathione ; Homeostasis ; Mitochondria ; N-Acetyl-L-cysteine ; Oxidative stress ; Reactive oxygen species
  • É parte de: Biological trace element research, 1901-01, Vol.127 (1), p.53-68
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    content type line 23
    ObjectType-Feature-1
  • Descrição: Cadmium (Cd) is a known nephrotoxic element. In this study, the primary cultures of rat proximal tubular (rPT) cells were treated with low doses of cadmium acetate (2.5 and 5 mu M) to investigate its cytotoxic mechanism. A progressive loss in cell viability, together with a significant increase in the number of apoptotic and necrotic cells, were seen in the experiment. Simultaneously, elevation of intracellular [Ca super(2+)]i and reactive oxygen species (ROS) levels, significant depletion of mitochondrial membrane potential(A V) and cellular glutathione (GSH), intracellular acidification, and inhibition of Na super(+), K super(+)-ATPase and Ca super(2+)-ATPase activities were revealed in a dose-dependent manner during the exposure, while the cellular death and the apoptosis could be markedly reversed by N-acetyl-L-cysteine (NAC). Also, the calcium overload and GSH depletion were significantly affected by NAC. In conclusion, exposure of rPT cells to low-dose cadmium led to cellular death, mediated by an apoptotic and a necrotic mechanism. The apoptotic death might be the chief mechanism, which may be mediated by oxidative stress. Also, a disorder of intracellular homeostasis induced by oxidative stress and mitochondrial dysfunction is a trigger of apoptosis in rPT cells.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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