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Proteomic and immunochemical characterization of glutathione transferase as a new allergen of the nematode Ascaris lumbricoides

Acevedo, Nathalie ; Mohr, Jens ; Zakzuk, Josefina ; Samonig, Martin ; Briza, Peter ; Erler, Anja ; Pomés, Anna ; Huber, Christian G ; Ferreira, Fatima ; Caraballo, Luis Bueno, Valquiria

PloS one, 2013-11, Vol.8 (11), p.e78353 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Proteomic and immunochemical characterization of glutathione transferase as a new allergen of the nematode Ascaris lumbricoides
Autor: Acevedo, Nathalie ; Mohr, Jens ; Zakzuk, Josefina ; Samonig, Martin ; Briza, Peter ; Erler, Anja ; Pomés, Anna ; Huber, Christian G ; Ferreira, Fatima ; Caraballo, Luis
Bueno, Valquiria
Assuntos: Adolescent ; Adult ; Allergenicity ; Allergens ; Allergens - chemistry ; Allergens - genetics ; Allergens - immunology ; Allergic reaction ; Allergies ; Allergy ; Amino Acid Sequence ; Amino Acid Substitution ; Amino acids ; Animals ; Antibodies ; Antigen-Antibody Reactions ; Antigens ; Antigens, Dermatophagoides - chemistry ; Antigens, Dermatophagoides - genetics ; Antigens, Dermatophagoides - immunology ; Ascaris ; Ascaris lumbricoides ; Ascaris lumbricoides - chemistry ; Ascaris lumbricoides - enzymology ; Ascaris lumbricoides - immunology ; Ascaris suum ; Asthma ; Asthma - genetics ; Asthma - immunology ; Blattaria ; Children ; Cloning ; Cockroaches ; Cockroaches - chemistry ; Cockroaches - immunology ; Colombia ; Comparative analysis ; Comparative studies ; Dermatophagoides pteronyssinus ; Diagnosis ; Dust ; Enzyme-linked immunosorbent assay ; Female ; Glutathione ; Glutathione transferase ; Glutathione Transferase - chemistry ; Glutathione Transferase - genetics ; Glutathione Transferase - immunology ; Goods and services tax ; Health aspects ; Helminth Proteins - chemistry ; Helminth Proteins - genetics ; Helminth Proteins - immunology ; House dust ; Humans ; Hypersensitivity ; Immunity ; Immunochemistry ; Immunoglobulin E ; Immunoglobulin E - immunology ; Immunoglobulins ; Immunology ; Infection ; Infections ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insect Proteins - immunology ; Isoenzymes - chemistry ; Isoenzymes - genetics ; Isoenzymes - immunology ; Isoforms ; Male ; Middle Aged ; Mites ; Models, Molecular ; Molecular biology ; Molecular Sequence Data ; Nematoda ; Onchocerca volvulus ; Parasites ; Parasitic diseases ; Pathogenesis ; Patients ; Protein Structure, Tertiary ; Proteomics ; Pyroglyphidae - chemistry ; Pyroglyphidae - immunology ; Rodents ; Roundworms ; Skin ; Thiols ; Tropical environment ; Tropical environments
É parte de: PloS one, 2013-11, Vol.8 (11), p.e78353
Notas: Conceived and designed the experiments: NA JM CGH FF LC. Performed the experiments: NA JM JZ MS PB AE. Analyzed the data: NA JM JZ MS PB LC. Contributed reagents/materials/analysis tools: JZ PB AE AP CGH FF LC. Wrote the paper: NA LC. Isolation of GSTs and immunochemical/glycodetection analysis: NA, JZ. 2D electrophoresis: NA, AE. Mass spectrometry measurements: JM, MS, PB. Supervised the intact masses analysis and Orbitrap measurements: CGH. Performed and supervised the MS analysis (QTOF): PB. Bioinformatic predicitions: JZ. Supervised the immunochemical characterization: FF LC. Overall responsability of the project: NA LC. Revised and contributed to the manuscript: NA JM JZ MS PB AE AP CGH FF LC.
Competing Interests: The author AP is employed by Indoor Biotechnologies, Inc. AP is the contact PI of an RO1 from the NIAID/NIH that partially finances this study. This does not alter the author's adherence to all the PLOS ONE policies on sharing data and materials. No competing interests exist for the rest of the authors.
Descrição: Helminth infections and allergy have evolutionary and clinical links. Infection with the nematode Ascaris lumbricoides induces IgE against several molecules including invertebrate pan-allergens. These antibodies influence the pathogenesis and diagnosis of allergy; therefore, studying parasitic and non-parasitic allergens is essential to understand both helminth immunity and allergy. Glutathione transferases (GSTs) from cockroach and house dust mites are clinically relevant allergens and comparative studies between them and the GST from A. lumbricoides (GSTA) are necessary to evaluate their allergenicity. We sought to analyze the allergenic potential of GSTA in connection with the IgE response to non-parasitic GSTs. IgE to purified GSTs from Ascaris (nGSTA and rGSTA), house dust mites (rDer p 8, nBlo t 8 and rBlo t 8), and cockroach (rBla g 5) was measured by ELISA in subjects from Cartagena, Colombia. Also, multidimensional proteomic approaches were used to study the extract of A. lumbricoides and investigate the existence of GST isoforms. We found that among asthmatics, the strength of IgE levels to GSTA was significantly higher than to mite and cockroach GSTs, and there was a strong positive correlation between IgE levels to these molecules. Specific IgE to GSTA was found in 13.2% of controls and 19.5% of asthmatics. In addition nGSTA induced wheal and flare in skin of sensitized asthmatics indicating that it might be of clinical relevance for some patients. Frequency and IgE levels to GSTA were higher in childhood and declined with age. At least six GST isoforms in A. lumbricoides bind human IgE. Four isoforms were the most abundant and several amino acid substitutions were found, mainly on the N-terminal domain. In conclusion, a new allergenic component of Ascaris has been discovered; it could have clinical impact in allergic patients and influence the diagnosis of mite and cockroach allergy in tropical environments.
Editor: United States: Public Library of Science
Idioma: Inglês

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Proteomic and immunochemical characterization of glutathione transferase as a new allergen of the nematode Ascaris lumbricoides

Acevedo, Nathalie ; Mohr, Jens ; Zakzuk, Josefina ; Samonig, Martin ; Briza, Peter ; Erler, Anja ; Pomés, Anna ; Huber, Christian G ; Ferreira, Fatima ; Caraballo, Luis Bueno, Valquiria

United States: Public Library of Science

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