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Circulating miR-17, miR-20a, miR-29c, and miR-223 combined as non-invasive biomarkers in nasopharyngeal carcinoma

Zeng, Xi ; Xiang, Juanjuan ; Wu, Minghua ; Xiong, Wei ; Tang, Hailin ; Deng, Min ; Li, Xiayu ; Liao, Qianjin ; Su, Bo ; Luo, Zhaohui ; Zhou, Yanhong ; Zhou, Ming ; Zeng, Zhaoyang ; Li, Xiaoling ; Shen, Shourong ; Shuai, Cijun ; Li, Guiyuan ; Fang, Jiasheng ; Peng, Shuping Peng, DunFa

PloS one, 2012-10, Vol.7 (10), p.e46367-e46367 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Circulating miR-17, miR-20a, miR-29c, and miR-223 combined as non-invasive biomarkers in nasopharyngeal carcinoma
Autor: Zeng, Xi ; Xiang, Juanjuan ; Wu, Minghua ; Xiong, Wei ; Tang, Hailin ; Deng, Min ; Li, Xiayu ; Liao, Qianjin ; Su, Bo ; Luo, Zhaohui ; Zhou, Yanhong ; Zhou, Ming ; Zeng, Zhaoyang ; Li, Xiaoling ; Shen, Shourong ; Shuai, Cijun ; Li, Guiyuan ; Fang, Jiasheng ; Peng, Shuping
Peng, DunFa
Assuntos: Analysis ; Bioindicators ; Biology ; Biomarkers ; Biomarkers, Tumor - blood ; Cancer ; Cancer diagnosis ; Carcinoma ; Case-Control Studies ; Cohort Studies ; Computed tomography ; Diagnosis ; Disease ; Gene expression ; Humans ; Infections ; Leukemia ; Lung cancer ; Lymphoma ; Mathematical models ; Medical diagnosis ; Medical prognosis ; Medical research ; Medicine ; MicroRNA ; MicroRNAs ; MicroRNAs - blood ; miRNA ; Nasopharyngeal carcinoma ; Nasopharyngeal Neoplasms - blood ; Patients ; Polymerase chain reaction ; Prognosis ; Proteins ; Real-Time Polymerase Chain Reaction ; Throat cancer
É parte de: PloS one, 2012-10, Vol.7 (10), p.e46367-e46367
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Undefined-2
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: GY SP. Performed the experiments: JJ MH HL XY QJ BS ZH YH M ZY. Analyzed the data: WX SR CJ. Contributed reagents/materials/analysis tools: SP GY. Wrote the paper: SP.
Descrição: MicroRNAs have been considered as a kind of potential novel biomarker for cancer detection due to their remarkable stability in the blood and the characteristics of their expression profile in many diseases. We performed microarray-based serum miRNA profiling on the serum of twenty nasopharyngeal carcinoma patients at diagnosis along with 20 non-cancerous individuals as controls. This was followed by a real-time quantitative Polymerase Chain Reaction (RT-qPCR) in a separate cohort of thirty patients with nasopharyngeal carcinoma and thirty age- matched non-cancerous volunteers. A model for diagnosis was established by a conversion of mathematical calculation formula which has been validated by analyzing 74 cases of patients with nasopharyngeal carcinoma and 57 cases of non-cancerous volunteers. The profiles showed that 39 and 17 miRNAs are exclusively expressed in the serum of non-cancerous volunteers and of patients with nasopharyngeal carcinoma respectively. 4 miRNAs including miR-17, miR-20a, miR-29c, and miR-223 were found to be expressed differentially in the serum of NPC compared with that of non-cancerous control. Based on this, a diagnosis equation with Ct difference method has been established to distinguish NPC cases and non-cancerous controls and validated with high sensitivity and specificity. We demonstrate that the serum miRNA-based biomarker model become a novel tool for NPC detection. The circulating 4-miRNA-based method may provide a novel strategy for NPC diagnosis.
Editor: United States: Public Library of Science
Idioma: Inglês

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Circulating miR-17, miR-20a, miR-29c, and miR-223 combined as non-invasive biomarkers in nasopharyngeal carcinoma

Zeng, Xi ; Xiang, Juanjuan ; Wu, Minghua ; Xiong, Wei ; Tang, Hailin ; Deng, Min ; Li, Xiayu ; Liao, Qianjin ; Su, Bo ; Luo, Zhaohui ; Zhou, Yanhong ; Zhou, Ming ; Zeng, Zhaoyang ; Li, Xiaoling ; Shen, Shourong ; Shuai, Cijun ; Li, Guiyuan ; Fang, Jiasheng ; Peng, Shuping Peng, DunFa

United States: Public Library of Science

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