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Atypical ‘HoBi’-like pestiviruses—Recent findings and implications thereof

Ståhl, K. ; Beer, M. ; Schirrmeier, H. ; Hoffmann, B. ; Belák, S. ; Alenius, S.

Veterinary microbiology, 2010-04, Vol.142 (1-2), p.90-93 [Periódico revisado por pares]

Amsterdam: Elsevier B.V

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Título:
Atypical ‘HoBi’-like pestiviruses—Recent findings and implications thereof
Autor: Ståhl, K. ; Beer, M. ; Schirrmeier, H. ; Hoffmann, B. ; Belák, S. ; Alenius, S.
Assuntos: 'HoBi'-like pestiviruses ; Animal and Dairy Science ; Animals ; Biological and medical sciences ; Bovine viral diarrhea virus 1 ; Bovine viral diarrhea virus 2 ; BVDV ; calves ; cattle ; Cell Culture Techniques ; Contamination ; Culture Media ; FCS ; fetal calf serum ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; geographical distribution ; HoBi ; Husdjursvetenskap ; literature reviews ; microbial contamination ; microbial genetics ; Microbiology ; Miscellaneous ; molecular sequence data ; nucleotide sequences ; Pestivirus ; Pestivirus - classification ; Pestivirus - genetics ; Pestivirus - isolation & purification ; Phylogeny ; polymerase chain reaction ; strain differences ; Veterinary Science ; Veterinärmedicin ; Virology
É parte de: Veterinary microbiology, 2010-04, Vol.142 (1-2), p.90-93
Notas: http://dx.doi.org/10.1016/j.vetmic.2009.09.048
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Descrição: In 2004, an atypical pestivirus named D32/00_‘HoBi’, isolated from foetal calf serum (FCS) originating from Brazil, was described (Schirrmeier et al., 2004). A few years later, a closely related virus (Th/04_KhonKaen) was detected in serum from a calf in Thailand, indicating that this group of atypical pestiviruses already is spread in cattle populations in various regions of the world. At the Friedrich-Loeffler-Institute, Insel Riems, Germany, FCS batches are regularly tested for pestivirus contamination, in general with positive PCR results, and in some cases the contaminants have been typed as ‘HoBi’-like. At the National Veterinary Institute (SVA) in Uppsala, Sweden, a recent event with contaminated FCS ruined much of the ongoing cell culture work. From the FCS and the contaminated cells we were able to amplify and sequence nucleic acid from three different pestivirus strains, including BVDV-1, -2 and ‘HoBi’-like; this in a commercial FCS that had been tested free from pestivirus by the manufacturer. In this short communication we review the current status of atypical ‘HoBi’-like pestiviruses, describe recent findings and discuss the implications thereof.
Editor: Amsterdam: Elsevier B.V
Idioma: Inglês

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Atypical ‘HoBi’-like pestiviruses—Recent findings and implications thereof

Ståhl, K. ; Beer, M. ; Schirrmeier, H. ; Hoffmann, B. ; Belák, S. ; Alenius, S.

Amsterdam: Elsevier B.V

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