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Albumin Church Bay: 560 Lys→Glu a new mutation detected by electrospray ionisation mass spectrometry

Chua, Evan K.M ; Brennan, Stephen O ; George, Peter M

Biochimica et biophysica acta, 1998-02, Vol.1382 (2), p.305-310 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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Título:
Albumin Church Bay: 560 Lys→Glu a new mutation detected by electrospray ionisation mass spectrometry
Autor: Chua, Evan K.M ; Brennan, Stephen O ; George, Peter M
Assuntos: Albumin Church Bay ; Amino Acid Sequence ; Amino acid substitution ; Chymotrypsin - metabolism ; Formylation ; Heterozygote ; Humans ; Mass Spectrometry ; Molecular Sequence Data ; Peptide Fragments - chemistry ; Peptide Mapping ; Point mutation ; Point Mutation - genetics ; Sequence Analysis, DNA ; Serum albumin ; Serum Albumin - chemistry ; Serum Albumin - genetics ; Serum Albumin, Human ; Trypsin - metabolism
É parte de: Biochimica et biophysica acta, 1998-02, Vol.1382 (2), p.305-310
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Albumin Church Bay is a fast migrating genetic variant of human serum albumin which, in a heterozygous subject, formed about 50% of the circulating albumin. Reversed phase peptide mapping and electrospray ionisation mass spectrometry (ESI-MS) indicated that the C-terminal CNBr peptide had decreased polarity associated with a 1 Da increase in mass. Subdigestion of this peptide with trypsin and chymotrypsin revealed that the increased mass was associated with the chymotrypsin fragment VEKCCKADDKETCF (555–568) which had a mass of 1791.1 compared to 1790.2 for its normal counterpart. Sequence analysis of PCR-amplified DNA indicated an A→G mutation at position 98 of exon 13, which causes a point mutation of 560 Lys→Glu and results in a 1 Da mass increase.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

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Albumin Church Bay: 560 Lys→Glu a new mutation detected by electrospray ionisation mass spectrometry

Chua, Evan K.M ; Brennan, Stephen O ; George, Peter M

Netherlands: Elsevier B.V

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