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Dynamics of bone marrow-derived endothelial progenitor cell/mesenchymal stem cell interaction in co-culture and its implications in angiogenesis

Aguirre, A. ; Planell, J.A. ; Engel, E.

Biochemical and biophysical research communications, 2010-09, Vol.400 (2), p.284-291 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Dynamics of bone marrow-derived endothelial progenitor cell/mesenchymal stem cell interaction in co-culture and its implications in angiogenesis
Autor: Aguirre, A. ; Planell, J.A. ; Engel, E.
Assuntos: 60 APPLIED LIFE SCIENCES ; ANGIOGENESIS ; APOPTOSIS ; BONE MARROW ; Bone Marrow Cells - cytology ; Bone Marrow Cells - physiology ; Cell Communication ; Co-culture ; Coculture Techniques ; Endothelial progenitor cell ; Endothelium, Vascular - cytology ; Endothelium, Vascular - physiology ; ENGINEERING ; GENES ; HOST ; Humans ; IMPLANTS ; IN VITRO ; IN VIVO ; INTERACTIONS ; LYMPHOKINES ; Mesenchymal stem cell ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Neovascularization, Physiologic ; POLYMERASE CHAIN REACTION ; REGULATIONS ; STEM CELLS ; Tissue Engineering ; Transport Vesicles
É parte de: Biochemical and biophysical research communications, 2010-09, Vol.400 (2), p.284-291
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: ► BM-EPCs and MSCs establish complex, self-organizing structures in co-culture. ► Co-culture decreases proliferation by cellular self-regulatory mechanisms. ► Co-cultured cells present an activated proangiogenic phenotype. ► qRT-PCR and cluster analysis identify new target genes playing important roles. Tissue engineering aims to regenerate tissues and organs by using cell and biomaterial-based approaches. One of the current challenges in the field is to promote proper vascularization in the implant to prevent cell death and promote host integration. Bone marrow endothelial progenitor cells (BM-EPCs) and mesenchymal stem cells (MSCs) are bone marrow resident stem cells widely employed for proangiogenic applications. In vivo, they are likely to interact frequently both in the bone marrow and at sites of injury. In this study, the physical and biochemical interactions between BM-EPCs and MSCs in an in vitro co-culture system were investigated to further clarify their roles in vascularization. BM-EPC/MSC co-cultures established close cell–cell contacts soon after seeding and self-assembled to form elongated structures at 3 days. Besides direct contact, cells also exhibited vesicle transport phenomena. When co-cultured in Matrigel, tube formation was greatly enhanced even in serum-starved, growth factor free medium. Both MSCs and BM-EPCs contributed to these tubes. However, cell proliferation was greatly reduced in co-culture and morphological differences were observed. Gene expression and cluster analysis for wide panel of angiogenesis-related transcripts demonstrated up-regulation of angiogenic markers but down-regulation of many other cytokines. These data suggest that cross-talk occurs in between BM-EPCs and MSCs through paracrine and direct cell contact mechanisms leading to modulation of the angiogenic response.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Dynamics of bone marrow-derived endothelial progenitor cell/mesenchymal stem cell interaction in co-culture and its implications in angiogenesis

Aguirre, A. ; Planell, J.A. ; Engel, E.

United States: Elsevier Inc

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