Idioma:

Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography

Bauda, Elda ; Gallet, Benoit ; Moravcova, Jana ; Effantin, Gregory ; Chan, Helena ; Novacek, Jiri ; Jouneau, Pierre-Henri ; Rodrigues, Christopher D A ; Schoehn, Guy ; Moriscot, Christine ; Morlot, Cecile

Nature communications, 2024-02, Vol.15 (1), p.1376-1376 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography
Autor: Bauda, Elda ; Gallet, Benoit ; Moravcova, Jana ; Effantin, Gregory ; Chan, Helena ; Novacek, Jiri ; Jouneau, Pierre-Henri ; Rodrigues, Christopher D A ; Schoehn, Guy ; Moriscot, Christine ; Morlot, Cecile
Assuntos: Bacillus subtilis - metabolism ; Bacteria ; Bacterial Proteins - metabolism ; Chromosomes ; Electron density ; Electron Microscope Tomography ; Fibers ; Ion beams ; Lamellae ; Life Sciences ; Macromolecules ; Micromachining ; Microscopy, Electron, Transmission ; Molecular properties ; Molecular structure ; Protein structure ; Proteins ; Quaternary structure ; Sporangia ; Spores ; Spores, Bacterial - genetics ; Sporulation ; Tomography ; Transmission electron microscopy ; Ultrastructure
É parte de: Nature communications, 2024-02, Vol.15 (1), p.1376-1376
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Bacterial spores owe their incredible resistance capacities to molecular structures that protect the cell content from external aggressions. Among the determinants of resistance are the quaternary structure of the chromosome and an extracellular shell made of proteinaceous layers (the coat), the assembly of which remains poorly understood. Here, in situ cryo-electron tomography on lamellae generated by cryo-focused ion beam micromachining provides insights into the ultrastructural organization of Bacillus subtilis sporangia. The reconstructed tomograms reveal that early during sporulation, the chromosome in the forespore adopts a toroidal structure harboring 5.5-nm thick fibers. At the same stage, coat proteins at the surface of the forespore form a stack of amorphous or structured layers with distinct electron density, dimensions and organization. By analyzing mutant strains using cryo-electron tomography and transmission electron microscopy on resin sections, we distinguish seven nascent coat regions with different molecular properties, and propose a model for the contribution of coat morphogenetic proteins.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography

Bauda, Elda ; Gallet, Benoit ; Moravcova, Jana ; Effantin, Gregory ; Chan, Helena ; Novacek, Jiri ; Jouneau, Pierre-Henri ; Rodrigues, Christopher D A ; Schoehn, Guy ; Moriscot, Christine ; Morlot, Cecile

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.