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Chemotherapeutic Targets in Osteosarcoma: Insights from Synchrotron-MicroFTIR and Quasi-Elastic Neutron Scattering

Marques, Maria Paula M ; Batista de Carvalho, Ana L. M ; Mamede, Adriana P ; Santos, Inês P ; García Sakai, Victoria ; Dopplapudi, Asha ; Cinque, Gianfelice ; Wolna, Magda ; Gardner, Peter ; Batista de Carvalho, Luís A. E

The journal of physical chemistry. B, 2019-08, Vol.123 (32), p.6968-6979 [Periódico revisado por pares]

United States: American Chemical Society

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  • Título:
    Chemotherapeutic Targets in Osteosarcoma: Insights from Synchrotron-MicroFTIR and Quasi-Elastic Neutron Scattering
  • Autor: Marques, Maria Paula M ; Batista de Carvalho, Ana L. M ; Mamede, Adriana P ; Santos, Inês P ; García Sakai, Victoria ; Dopplapudi, Asha ; Cinque, Gianfelice ; Wolna, Magda ; Gardner, Peter ; Batista de Carvalho, Luís A. E
  • É parte de: The journal of physical chemistry. B, 2019-08, Vol.123 (32), p.6968-6979
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: This study aimed at the development of improved drugs against human osteosarcoma, which is the most common primary bone tumor in children and teenagers with a low prognosis. New insights into the impact of an unconventional Pd­(II) anticancer agent on human osteosarcoma cells were obtained by synchrotron radiation-Fourier transform infrared microspectroscopy and quasi-elastic neutron scattering (QENS) experiments from its effect on the cellular metabolism to its influence on intracellular water, which can be regarded as a potential secondary pharmacological target. Specific infrared biomarkers of drug action were identified, enabling a molecular-level description of variations in cellular biochemistry upon drug exposure. The main changes were detected in the protein and lipid cellular components, namely, in the ratio of unsaturated-to-saturated fatty acids. QENS revealed reduced water mobility within the cytoplasm for drug-treated cells, coupled to a disruption of the hydration layers of biomolecules. Additionally, the chemical and dynamical profiles of osteosarcoma cells were compared to those of metastatic breast cancer cells, revealing distinct dissimilarities that may influence drug activity.
  • Editor: United States: American Chemical Society
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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