skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

A Comprehensive Proteomic Analysis of the Type III Secretome of Citrobacter rodentium

Deng, Wanyin ; de Hoog, Carmen L. ; Yu, Hong B. ; Li, Yuling ; Croxen, Matthew A. ; Thomas, Nikhil A. ; Puente, Jose L. ; Foster, Leonard J. ; Finlay, B. Brett

The Journal of biological chemistry, 2010-02, Vol.285 (9), p.6790-6800 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por
  • Título:
    A Comprehensive Proteomic Analysis of the Type III Secretome of Citrobacter rodentium
  • Autor: Deng, Wanyin ; de Hoog, Carmen L. ; Yu, Hong B. ; Li, Yuling ; Croxen, Matthew A. ; Thomas, Nikhil A. ; Puente, Jose L. ; Foster, Leonard J. ; Finlay, B. Brett
  • Assuntos: Animals ; Bacteria ; Bacterial Proteins - analysis ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Citrobacter rodentium ; Citrobacter rodentium - chemistry ; Effectors ; Escherichia coli ; Gene Deletion ; Genomics and Proteomics ; Infections - microbiology ; Mass Spectrometry (MS) ; Mass Spectrometry - methods ; Mice ; Protein Export ; Protein Secretion ; Proteomics ; Proteomics - methods ; Quantitative Proteomics ; Secretome ; Secretory Pathway ; Type III Secretion System
  • É parte de: The Journal of biological chemistry, 2010-02, Vol.285 (9), p.6790-6800
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-1
    content type line 23
    Supported by a Canadian Institutes of Health Research postdoctoral fellowship.
    Present address: Centre for High-Throughput Biology and Dept. of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
    Supported by a Canadian Institutes of Health Research/Canadian Association of Gastroenterology/Ferring Pharmaceuticals postdoctoral fellowship.
  • Descrição: Enteropathogenic Escherichia coli, enterohemorrhagic E. coli, and Citrobacter rodentium belong to the family of attaching and effacing (A/E) bacterial pathogens. They intimately attach to host intestinal epithelial cells, trigger the effacement of intestinal microvilli, and cause diarrheal disease. Central to their pathogenesis is a type III secretion system (T3SS) encoded by a pathogenicity island called the locus of enterocyte effacement (LEE). The T3SS is used to inject both LEE- and non-LEE-encoded effector proteins into the host cell, where these effectors modulate host signaling pathways and immune responses. Identifying the effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. Here we analyzed the type III secretome of C. rodentium using the highly sensitive and quantitative SILAC (stable isotope labeling with amino acids in cell culture)-based mass spectrometry. This approach not only confirmed nearly all known secreted proteins and effectors previously identified by conventional biochemical and proteomic techniques, but also identified several new secreted proteins. The T3SS-dependent secretion of these new proteins was validated, and five of them were translocated into cultured cells, representing new or additional effectors. Deletion mutants for genes encoding these effectors were generated in C. rodentium and tested in a murine infection model. This study comprehensively characterizes the type III secretome of C. rodentium, expands the repertoire of type III secreted proteins and effectors for the A/E pathogens, and demonstrates the simplicity and sensitivity of using SILAC-based quantitative proteomics as a tool for identifying substrates for protein secretion systems.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.