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Efficiency of microspore culture for doubled haploid production in the breeding project "Czech Winter Rape"

Smykalova, I.(Agritec, Sumperk (Czech Republic)) E-mail:smykalova@agritec.cz ; Vetrovcova, M.(Agritec, Sumperk (Czech Republic)) ; Klima, M.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic). Odbor Genetiky a Slechteni) ; Machackova, I.(Selgen, Chlumec nad Cidlinou (Czech Republic)) ; Griga, M.(Agritec, Sumperk (Czech Republic))

Czech Journal of Genetics and Plant Breeding, 2006-06, Vol.42 (2), p.58-71 [Periódico revisado por pares]

Prague: Czech Academy of Agricultural Sciences (CAAS)

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Título:
Efficiency of microspore culture for doubled haploid production in the breeding project "Czech Winter Rape"
Autor: Smykalova, I.(Agritec, Sumperk (Czech Republic)) E-mail:smykalova@agritec.cz ; Vetrovcova, M.(Agritec, Sumperk (Czech Republic)) ; Klima, M.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic). Odbor Genetiky a Slechteni) ; Machackova, I.(Selgen, Chlumec nad Cidlinou (Czech Republic)) ; Griga, M.(Agritec, Sumperk (Czech Republic))
Assuntos: AMELIORATION DES PLANTES ; Brassica ; BRASSICA NAPUS ; brassica napus l ; Breeding ; COLCHICINA ; COLCHICINE ; Cotyledons ; CULTIVOS DE INVIERNO ; CULTURE D'HIVER ; CULTURE MEDIA ; DESARROLLO EMBRIONARIO ; DEVELOPPEMENT EMBRYONNAIRE ; Diploids ; doubled haploids ; EMBRIONES VEGETALES ; EMBRYON VEGETAL ; EMBRYONIC DEVELOPMENT ; Embryos ; F1 HYBRIDS ; FERTILIDAD ; FERTILITE ; FERTILITY ; FITOMEJORAMIENTO ; GENOTIPOS ; GENOTYPE ; GENOTYPES ; Germination ; GRAINE DE COLZA ; HAPLOIDIA ; HAPLOIDIE ; HAPLOIDY ; HIBRIDOS F1 ; hybrid breeding ; HYBRIDE F1 ; Hybrids ; IN VITRO REGENERATION ; MEDIO DE CULTIVO ; microspore culture ; MILIEU DE CULTURE ; Morphology ; PLANT BREEDING ; PLANT EMBRYOS ; Plantlets ; POLIPLOIDIA ; POLYPLOIDIE ; POLYPLOIDY ; Progeny ; Rape ; RAPESEED ; REGENERACION IN VITRO ; REGENERATION IN VITRO ; Rooting ; Seeds ; SEMILLA DE COLZA ; Shoots ; Sterility ; Winter ; WINTER CROPS ; winter rape
É parte de: Czech Journal of Genetics and Plant Breeding, 2006-06, Vol.42 (2), p.58-71
Notas: F02
http://www.cazv.cz/UserFiles/File/CJGPB%2042_58-71.pdf
F30
2006000672
Descrição: Twenty interline F1 hybrids of Brassica napus were evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in a breeding project. All tested genotypes were able to produce microspore-derived embryos. Nevertheless, significant differences were found in the frequency of embryo production as related to particular genotypes. However, fertile DH regenerants were obtained only in 12 hybrid lines. Colchicine treatment induced diploid (doubled haploid), polyploid (triploid, tetraploid) and mixoploid (containing both euploid and aneuploid nuclei) plants. In total, 590 microspore-derived regenerants were produced in a series of experiments, 236 of them represented fully fertile DH plants. The R2 seeds (progeny of DH R1-generation) of the most promising hybrid lines were included in the tests of agronomic performance in field conditions.
Editor: Prague: Czech Academy of Agricultural Sciences (CAAS)
Idioma: Inglês;Tcheco;Eslovaco

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Efficiency of microspore culture for doubled haploid production in the breeding project "Czech Winter Rape"

Prague: Czech Academy of Agricultural Sciences (CAAS)

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