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Expression and Protective Role of Heme Oxygenase-1 in Delayed Myocardial Preconditioning

JANCSÓ, GÁBOR ; CSEREPES, BARBARA ; GASZ, BALÁZS ; BENKÓ, LÁSZLÓ ; BORSICZKY, BALÁZS ; FERENC, ANDREA ; KÜRTHY, MÁRIA ; RÁCZ, BOGLÁRKA ; LANTOS, JÁNOS ; GÁL, JÁNOS ; ARATÓ, ENDRE ; SÍNAYC, LÓSZLÓ ; WÉBER, GYÖRGY ; RÓTH, ERZSÉBET

Annals of the New York Academy of Sciences, 2007-01, Vol.1095 (1), p.251-261 [Periódico revisado por pares]

Malden, USA: Blackwell Publishing Inc

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Título:
Expression and Protective Role of Heme Oxygenase-1 in Delayed Myocardial Preconditioning
Autor: JANCSÓ, GÁBOR ; CSEREPES, BARBARA ; GASZ, BALÁZS ; BENKÓ, LÁSZLÓ ; BORSICZKY, BALÁZS ; FERENC, ANDREA ; KÜRTHY, MÁRIA ; RÁCZ, BOGLÁRKA ; LANTOS, JÁNOS ; GÁL, JÁNOS ; ARATÓ, ENDRE ; SÍNAYC, LÓSZLÓ ; WÉBER, GYÖRGY ; RÓTH, ERZSÉBET
Assuntos: Animals ; Animals, Newborn ; Cells, Cultured ; heme oxygenase ; Heme Oxygenase-1 - biosynthesis ; Heme Oxygenase-1 - genetics ; Heme Oxygenase-1 - physiology ; Ischemic Preconditioning, Myocardial ; myocardium ; Myocardium - cytology ; Myocardium - enzymology ; preconditioning ; Rats ; Rats, Wistar
É parte de: Annals of the New York Academy of Sciences, 2007-01, Vol.1095 (1), p.251-261
Notas: istex:FF24901AC1E3F6202B2EFFDBBED350EDA1D4ED89
ark:/67375/WNG-JDK9M30Q-J
ArticleID:NYAS29
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
Descrição: : In the study the authors aimed to demonstrate the expression and protective effect of heme oxygenase‐1 (HO‐1) in the delayed preconditioning (PC) on cultured myocardiac cells. Neonatal rat cardiac myocytes were exposed to ischemic (ischemic medium [IM] for 20 min) and pharmacological (adenosine, epinephrine, opioid) PC. Twenty‐four hours later cells were subjected to a simulated ischemia (SI )—culturing for 3 h in IM, followed by 2‐h reperfusion in normal medium‐–and then lactate dehydrogenase (LDH), live/death ratio, and apoptosis were measured. For demonstrating the protective role of HO‐1, its enzymatic activity was competitively inhibited by administration of zinc protoporphyrin IX (ZnPPIX), and HO‐1 synthesis was blocked with HO‐1 siRNA. Cells in control group were cultured under normoxic conditions. In SI group, cells underwent only an SI without PC. HO‐1 expression in all of the groups was demonstrated with immunostaining. Our results showed a significant decrease of LDH release, apoptosis, and cell death in PC groups versus SI group, which has been risen in ZnPPIX‐ and HO‐1 siRNA‐treated groups. HO‐1 immunostaining showed an appreciable HO‐1 expression in PC groups, which was abolished with HO‐1 siRNA administration, but not in ZnPPIX group. The results therefore suggest that HO‐1 expression increases in both ischemic and pharmacological PC, and HO‐1 has cellular protective effect against cell death and apoptosis in ischemia‐reperfusion‐induced oxidative injury.
Editor: Malden, USA: Blackwell Publishing Inc
Idioma: Inglês

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Expression and Protective Role of Heme Oxygenase-1 in Delayed Myocardial Preconditioning

JANCSÓ, GÁBOR ; CSEREPES, BARBARA ; GASZ, BALÁZS ; BENKÓ, LÁSZLÓ ; BORSICZKY, BALÁZS ; FERENC, ANDREA ; KÜRTHY, MÁRIA ; RÁCZ, BOGLÁRKA ; LANTOS, JÁNOS ; GÁL, JÁNOS ; ARATÓ, ENDRE ; SÍNAYC, LÓSZLÓ ; WÉBER, GYÖRGY ; RÓTH, ERZSÉBET

Malden, USA: Blackwell Publishing Inc

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