Idioma:

Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach

Carr, Steven A. ; Abbatiello, Susan E. ; Ackermann, Bradley L. ; Borchers, Christoph ; Domon, Bruno ; Deutsch, Eric W. ; Grant, Russell P. ; Hoofnagle, Andrew N. ; Hüttenhain, Ruth ; Koomen, John M. ; Liebler, Daniel C. ; Liu, Tao ; MacLean, Brendan ; Mani, DR ; Mansfield, Elizabeth ; Neubert, Hendrik ; Paulovich, Amanda G. ; Reiter, Lukas ; Vitek, Olga ; Aebersold, Ruedi ; Anderson, Leigh ; Bethem, Robert ; Blonder, Josip ; Boja, Emily ; Botelho, Julianne ; Boyne, Michael ; Bradshaw, Ralph A. ; Burlingame, Alma L. ; Chan, Daniel ; Keshishian, Hasmik ; Kuhn, Eric ; Kinsinger, Christopher ; Lee, Jerry S.H. ; Lee, Sang-Won ; Moritz, Robert ; Oses-Prieto, Juan ; Rifai, Nader ; Ritchie, James ; Rodriguez, Henry ; Srinivas, Pothur R. ; Townsend, R. Reid ; Van Eyk, Jennifer ; Whiteley, Gordon ; Wiita, Arun ; Weintraub, Susan

Molecular & cellular proteomics, 2014-03, Vol.13 (3), p.907-917 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach
Autor: Carr, Steven A. ; Abbatiello, Susan E. ; Ackermann, Bradley L. ; Borchers, Christoph ; Domon, Bruno ; Deutsch, Eric W. ; Grant, Russell P. ; Hoofnagle, Andrew N. ; Hüttenhain, Ruth ; Koomen, John M. ; Liebler, Daniel C. ; Liu, Tao ; MacLean, Brendan ; Mani, DR ; Mansfield, Elizabeth ; Neubert, Hendrik ; Paulovich, Amanda G. ; Reiter, Lukas ; Vitek, Olga ; Aebersold, Ruedi ; Anderson, Leigh ; Bethem, Robert ; Blonder, Josip ; Boja, Emily ; Botelho, Julianne ; Boyne, Michael ; Bradshaw, Ralph A. ; Burlingame, Alma L. ; Chan, Daniel ; Keshishian, Hasmik ; Kuhn, Eric ; Kinsinger, Christopher ; Lee, Jerry S.H. ; Lee, Sang-Won ; Moritz, Robert ; Oses-Prieto, Juan ; Rifai, Nader ; Ritchie, James ; Rodriguez, Henry ; Srinivas, Pothur R. ; Townsend, R. Reid ; Van Eyk, Jennifer ; Whiteley, Gordon ; Wiita, Arun ; Weintraub, Susan
Assuntos: Animals ; Biological Assay - methods ; Biology ; Environmental Molecular Sciences Laboratory ; Guidelines as Topic ; Humans ; Isotope Labeling ; Mass Spectrometry - methods ; Medicine ; Peptides - metabolism ; Proteomics - standards ; Reference Standards ; Software ; Technological Innovation and Resources
É parte de: Molecular & cellular proteomics, 2014-03, Vol.13 (3), p.907-917
Notas: ObjectType-Article-1
content type line 25
ObjectType-Conference-2
SourceType-Conference Papers & Proceedings-1
USDOE
AC05-76RL01830
PNNL-SA-102320
Descrição: Adoption of targeted mass spectrometry (MS) approaches such as multiple reaction monitoring (MRM) to study biological and biomedical questions is well underway in the proteomics community. Successful application depends on the ability to generate reliable assays that uniquely and confidently identify target peptides in a sample. Unfortunately, there is a wide range of criteria being applied to say that an assay has been successfully developed. There is no consensus on what criteria are acceptable and little understanding of the impact of variable criteria on the quality of the results generated. Publications describing targeted MS assays for peptides frequently do not contain sufficient information for readers to establish confidence that the tests work as intended or to be able to apply the tests described in their own labs. Guidance must be developed so that targeted MS assays with established performance can be made widely distributed and applied by many labs worldwide. To begin to address the problems and their solutions, a workshop was held at the National Institutes of Health with representatives from the multiple communities developing and employing targeted MS assays. Participants discussed the analytical goals of their experiments and the experimental evidence needed to establish that the assays they develop work as intended and are achieving the required levels of performance. Using this “fit-for-purpose” approach, the group defined three tiers of assays distinguished by their performance and extent of analytical characterization. Computational and statistical tools useful for the analysis of targeted MS results were described. Participants also detailed the information that authors need to provide in their manuscripts to enable reviewers and readers to clearly understand what procedures were performed and to evaluate the reliability of the peptide or protein quantification measurements reported. This paper presents a summary of the meeting and recommendations.
Editor: United States: Elsevier Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach

United States: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.