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Artefacts in cell culture: Pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media

Long, Lee Hua ; Halliwell, Barry

Biochemical and biophysical research communications, 2009-10, Vol.388 (4), p.700-704 [Periódico revisado por pares]

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Título:
Artefacts in cell culture: Pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media
Autor: Long, Lee Hua ; Halliwell, Barry
Assuntos: Ascorbate ; Ascorbic Acid - metabolism ; Catechin - analogs & derivatives ; Catechin - metabolism ; Cell culture ; Culture Media - metabolism ; Dulbecco’s modified Eagle’s medium ; Epigallocatechin gallate ; Free Radical Scavengers - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Pyruvate ; Pyruvic Acid - metabolism
É parte de: Biochemical and biophysical research communications, 2009-10, Vol.388 (4), p.700-704
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, media containing pyruvate showed much less H 2O 2 production, apparently because pyruvate can scavenge H 2O 2 in the medium. Researchers must be aware that compounds under test can sometimes readily oxidise in cell culture media, that this might not be detected by measurement of H 2O 2 if the media contain pyruvate, and that pyruvate can be substantially depleted in the media as a result.
Editor: United States: Elsevier Inc
Idioma: Inglês

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