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Genotyping of Salmonella enterica strains from animal and human origin using three molecular techniques

Cruz-Méndez, Juan S. ; Ortiz-Muñoz, Julián D. ; Rondon-Barragan, Iang S.

Iraqi journal of veterinary sciences, 2022-03, Vol.36 (2), p.531-538 [Periódico revisado por pares]

University of Mosul, College of Veterinary Medicine

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  • Título:
    Genotyping of Salmonella enterica strains from animal and human origin using three molecular techniques
  • Autor: Cruz-Méndez, Juan S. ; Ortiz-Muñoz, Julián D. ; Rondon-Barragan, Iang S.
  • Assuntos: genetic profile ; phylogeny ; salmonellosis ; serotypes
  • É parte de: Iraqi journal of veterinary sciences, 2022-03, Vol.36 (2), p.531-538
  • Descrição: This study aims to characterize different Salmonella enterica subsp molecularly. enterica strains (n=49) were isolated from human gastrointestinal cases in the Tolima region and poultry from Santander and Tolima regions using PCR-RFLP, PCR-ribotyping, and PCR-SSCP. The band patterns obtained with each technique were analyzed by building dendrograms based on the Unweighted Pair Group Method with Arithmetic mean (UPGMA) method and using the Dice coefficient. On the other hand, the discriminatory power of each technique was assessed using Simpson's discriminatory index. The genetic profiles of the gnd gene obtained with AciI restriction enzyme and the PCR-SSCP carried out with groEL gene allowed the inter-and intraserovar differentiation. Finally, the PCR-ribotyping method exhibited the highest discriminatory power (0.8571). In conclusion, we show three PCR-based genotyping methods providing an alternative for identifying similarities and differences within Salmonella enterica strains from different geographic and biological regions.
  • Editor: University of Mosul, College of Veterinary Medicine
  • Idioma: Inglês;Árabe
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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