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Comparative genomics of four lactic acid bacteria identified with Vitek MS (MALDI-TOF) and whole-genome sequencing

Kahraman-Ilıkkan, Özge

Molecular genetics and genomics : MGG, 2024-12, Vol.299 (1), p.31-31 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Comparative genomics of four lactic acid bacteria identified with Vitek MS (MALDI-TOF) and whole-genome sequencing
Autor: Kahraman-Ilıkkan, Özge
Assuntos: Animal Genetics and Genomics ; Antibiotic resistance ; Bacteria ; Bacteriocins ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; Biosynthesis ; CRISPR ; Fermentation ; Gene clusters ; Genes ; Genomes ; Genomics ; Human Genetics ; Lactic acid bacteria ; Lactiplantibacillus plantarum ; Lactobacillales ; Lactobacillus ; Lentilactobacillus ; Life Sciences ; Metabolites ; Microbial Genetics and Genomics ; Next-generation sequencing ; Original ; Original Article ; Plant Genetics and Genomics ; Probiotics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Starter cultures ; Whole Genome Sequencing
É parte de: Molecular genetics and genomics : MGG, 2024-12, Vol.299 (1), p.31-31
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
Communicated by Martine Collart.
Descrição: Lactic acid bacteria (LAB) can be used as a probiotic or starter culture in dairy, meat, and vegetable fermentation. Therefore, their isolation and identification are essential. Recent advances in omics technologies and high-throughput sequencing have made the identification and characterization of bacteria. This study firstly aimed to demonstrate the sensitivity of the Vitek MS (MALDI-TOF) system in the identification of lactic acid bacteria and, secondly, to characterize bacteria using various bioinformatics approaches. Probiotic potency-related genes and secondary metabolite biosynthesis gene clusters were examined. The Vitek MS (MALDI-TOF) system was able to identify all of the bacteria at the genus level. According to whole genome sequencing, the bacteria were confirmed to be Lentilactobacillus buchneri, Levilactobacillus brevis, Lactiplantibacillus plantarum, Levilactobacillus namurensis. Bacteria had most of the probiotic potency-related genes, and different toxin-antitoxin systems such as PemIK/MazEF, Hig A/B, YdcE/YdcD, YefM/YoeB. Also, some of the secondary metabolite biosynthesis gene clusters, some toxic metabolite-related genes, and antibiotic resistance-related genes were detected. In addition, Lentilactobacillus buchneri Egmn17 had a type II-A CRISPR/Cas system. Lactiplantibacillus plantarum Gmze16 had a bacteriocin, plantaricin E/F.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Comparative genomics of four lactic acid bacteria identified with Vitek MS (MALDI-TOF) and whole-genome sequencing

Kahraman-Ilıkkan, Özge

Berlin/Heidelberg: Springer Berlin Heidelberg

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