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Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle

Song, Zhe ; Moore, Daniel R ; Hodson, Nathan ; Ward, Carl ; Dent, Jessica R ; O'Leary, Mary F ; Shaw, Andrew M ; Hamilton, D Lee ; Sarkar, Sovan ; Gangloff, Yann-Gaël ; Hornberger, Troy A ; Spriet, Lawrence L ; Heigenhauser, George J ; Philp, Andrew

Scientific reports, 2017-07, Vol.7 (1), p.5028-14, Article 5028 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
Autor: Song, Zhe ; Moore, Daniel R ; Hodson, Nathan ; Ward, Carl ; Dent, Jessica R ; O'Leary, Mary F ; Shaw, Andrew M ; Hamilton, D Lee ; Sarkar, Sovan ; Gangloff, Yann-Gaël ; Hornberger, Troy A ; Spriet, Lawrence L ; Heigenhauser, George J ; Philp, Andrew
Assuntos: Adult ; Cell Membrane - metabolism ; Dietary Carbohydrates - administration & dosage ; Dietary Proteins - administration & dosage ; Eukaryotic Initiation Factor-3 - metabolism ; Female ; Humans ; Immunofluorescence ; Initiation factor eIF-3 ; Life Sciences ; Localization ; Lysosomal protein ; Lysosomal-Associated Membrane Protein 2 - metabolism ; Male ; mRNA ; Muscle Contraction ; Muscle, Skeletal - metabolism ; Musculoskeletal system ; Protein Binding ; Protein biosynthesis ; Protein Transport ; Proteins ; Rapamycin ; Ras Homolog Enriched in Brain Protein - metabolism ; Resistance Training - methods ; Skeletal muscle ; TOR protein ; TOR Serine-Threonine Kinases - metabolism ; Tuberous Sclerosis Complex 2 ; Tuberous Sclerosis Complex 2 Protein - metabolism ; Young Adult
É parte de: Scientific reports, 2017-07, Vol.7 (1), p.5028-14, Article 5028
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
PMCID: PMC5504043
Descrição: The mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly co-localised in basal samples. Resistance exercise resulted in rapid translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance exercise led to the dissociation of TSC2 from Rheb and increased in the co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F (eIF3F) at the cell membrane post-exercise in both groups, with the response significantly greater at 1 h of recovery in the FED compared to CON. Collectively our data demonstrate that cellular trafficking of mTOR occurs in human muscle in response to an anabolic stimulus, events that appear to be primarily influenced by muscle contraction. The translocation and association of mTOR with positive regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA translational capacity after resistance exercise.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle

Song, Zhe ; Moore, Daniel R ; Hodson, Nathan ; Ward, Carl ; Dent, Jessica R ; O'Leary, Mary F ; Shaw, Andrew M ; Hamilton, D Lee ; Sarkar, Sovan ; Gangloff, Yann-Gaël ; Hornberger, Troy A ; Spriet, Lawrence L ; Heigenhauser, George J ; Philp, Andrew

England: Nature Publishing Group

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