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Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex

Kadoshima, Taisuke ; Sakaguchi, Hideya ; Nakano, Tokushige ; Soen, Mika ; Ando, Satoshi ; Eiraku, Mototsugu ; Sasai, Yoshiki

Proceedings of the National Academy of Sciences - PNAS, 2013-12, Vol.110 (50), p.20284-20289 [Periódico revisado por pares]

Washington, DC: National Academy of Sciences

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Título:
Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex
Autor: Kadoshima, Taisuke ; Sakaguchi, Hideya ; Nakano, Tokushige ; Soen, Mika ; Ando, Satoshi ; Eiraku, Mototsugu ; Sasai, Yoshiki
Assuntos: Amides ; Biological and medical sciences ; Biological Sciences ; Brain ; Cell Culture Techniques ; Cell Polarity - physiology ; Cells ; Collagen ; Cultured cells ; Drug Combinations ; Embryonic Stem Cells - physiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Laminin ; Neocortex ; Neocortex - cytology ; Neocortex - physiology ; Neural stem cells ; Neuroglia ; Neuroglia - cytology ; Neurons ; Neuroscience ; Organogenesis - physiology ; Progenitor cells ; Proteoglycans ; Pyridines ; Rodents ; Species Specificity ; Stem cells ; Tissues ; Vertebrates: nervous system and sense organs
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2013-12, Vol.110 (50), p.20284-20289
Notas: http://dx.doi.org/10.1073/pnas.1315710110
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Author contributions: T.K. and Y.S. designed research; T.K., H.S., T.N., M.S., S.A., and M.E. performed research; T.K. and Y.S. analyzed data; and T.K. and Y.S. wrote the paper.
2Present address: Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka 554-8558, Japan.
Edited by Chen-Ming Fan, Carnegie Institution of Washington, Baltimore, MD, and accepted by the Editorial Board October 17, 2013 (received for review August 21, 2013)
1Present address: Faculty of Exploratory Pharmacology, Asubio Pharma Co., Ltd., Kobe 650-0047, Japan.
Descrição: Here, using further optimized 3D culture that allows highly selective induction and long-term growth of human ES cell (hESC)-derived cortical neuroepithelium, we demonstrate unique aspects of self-organization in human neocorticogenesis. Self-organized cortical tissue spontaneously forms a polarity along the dorsocaudal-ventrorostral axis and undergoes region-specific rolling morphogenesis that generates a semispherical structure. The neuroepithelium self-forms a multilayered structure including three neuronal zones (subplate, cortical plate, and Cajal-Retzius cell zones) and three progenitor zones (ventricular, subventricular, and intermediate zones) in the same apical-basal order as seen in the human fetal cortex in the early second trimester. In the cortical plate, late-born neurons tend to localize more basally to early-born neurons, consistent with the inside-out pattern seen in vivo. Furthermore, the outer subventricular zone contains basal progenitors that share characteristics with outer radial glia abundantly found in the human, but not mouse, fetal brain. Thus, human neocorticogenesis involves intrinsic programs that enable the emergence of complex neocortical features.
Editor: Washington, DC: National Academy of Sciences
Idioma: Inglês

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Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex

Kadoshima, Taisuke ; Sakaguchi, Hideya ; Nakano, Tokushige ; Soen, Mika ; Ando, Satoshi ; Eiraku, Mototsugu ; Sasai, Yoshiki

Washington, DC: National Academy of Sciences

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