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Distribution of Stable Free Radicals among Amino Acids of Isolated Soy Proteins

Lei, Qingxin ; Liebold, Christopher M ; Boatright, William L ; Shah Jahan, M

Journal of food science, 2010-09, Vol.75 (7), p.C633-C640 [Periódico revisado por pares]

Malden, USA: Blackwell Publishing Inc

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Título:
Distribution of Stable Free Radicals among Amino Acids of Isolated Soy Proteins
Autor: Lei, Qingxin ; Liebold, Christopher M ; Boatright, William L ; Shah Jahan, M
Assuntos: acid hydrolysis ; Amino acids ; Amino Acids - analysis ; Amino Acids - chemistry ; Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts ; Atmosphere Exposure Chambers ; Biochemistry ; Biological and medical sciences ; Derivatives ; Deuterium ; Deuterium - chemistry ; deuterium sulfide ; Electron Spin Resonance Spectroscopy ; Enrichment ; Food industries ; Food science ; Foods ; Formates - chemistry ; free radical distribution ; free radical quenching ; Free radicals ; Free Radicals - analysis ; Free Radicals - chemistry ; Fundamental and applied biological sciences. Psychology ; gas chromatography ; Gas Chromatography-Mass Spectrometry ; Hydrogen Sulfide - chemistry ; Hydrolysis ; Indicators and Reagents - chemistry ; isolated soy proteins ; Isotope Labeling ; isotope ratio ; Isotope ratios ; Isotopes ; Labels ; mass spectrometry ; Mesylates - chemistry ; Plant Proteins, Dietary - chemistry ; powders ; protein isolates ; Proteins ; soy protein ; Soybean Proteins - chemistry ; stable free radical ; Sulfide compounds ; sulfides
É parte de: Journal of food science, 2010-09, Vol.75 (7), p.C633-C640
Notas: http://dx.doi.org/10.1111/j.1750-3841.2010.01746.x
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Descrição: Application of deuterium sulfide to powdered isolated soy proteins (ISP) was used to quench stable free radicals and produce a single deuterium label on amino acids where free radicals reside. The deuterium labels rendered increases of isotope ratio for the specific ions of radical-bearing amino acids. Isotope ratio measurements were achieved by gas chromatography/mass spectrometry (GC/MS) analyses after the amino acids were released by acidic hydrolysis and converted to volatile derivatives with propyl chloroformate. The isotope enrichment data showed the stable free radicals were located on Ala, Gly, Leu, Ile, Asx (Asp+Asn), Glx (Glu+Gln), and Trp but not on Val, Pro, Met, Phe, Lys, and His. Due to the low abundance of Ser, Thr, and Cys derivatives and the impossibility to accurately measure their isotope ratios, the radical bearing status for these amino acids remained undetermined even though their derivatives were positively identified from ISP hydrolysates. The relative isotope enrichment for radical-bearing amino acids Ala, Gly, Leu, Ile, Asx (Asp+Asn), Glx (Glu+Gln), and Trp were 8.67%, 2.96%, 2.90%, 3.94%, 6.03%, 3.91%, and 21.48%, respectively. Isotope ratio increase for Tyr was also observed but further investigation revealed such increase was mainly from nonspecific deuterium-hydrogen exchange not free radical quenching. The results obtained from the present study provide important information for a better understanding of the mechanisms of free radical formation and stabilization in "dry" ISP.
Editor: Malden, USA: Blackwell Publishing Inc
Idioma: Inglês

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Distribution of Stable Free Radicals among Amino Acids of Isolated Soy Proteins

Lei, Qingxin ; Liebold, Christopher M ; Boatright, William L ; Shah Jahan, M

Malden, USA: Blackwell Publishing Inc

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