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The 5′ end group of tobacco mosaic virus RNA is m7G5′ppp5′Gp

Zimmern, D

Nucleic acids research, 1975-07, Vol.2 (7), p.1189-1202 [Periódico revisado por pares]

England: Oxford University Press

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Título:
The 5′ end group of tobacco mosaic virus RNA is m7G5′ppp5′Gp
Autor: Zimmern, D
Assuntos: Alkaline Phosphatase ; Animals ; Base Sequence ; Chromatography, Thin Layer ; Coliphages - enzymology ; Escherichia coli - enzymology ; Guanine Nucleotides - analysis ; Nucleotides ; Pancreas - enzymology ; Phosphoric Diester Hydrolases ; Pyrophosphatases ; Ribonucleases ; RNA, Viral - analysis ; RNA, Viral - isolation & purification ; Snakes ; Tobacco mosaic virus ; Tobacco Mosaic Virus - analysis ; Venoms
É parte de: Nucleic acids research, 1975-07, Vol.2 (7), p.1189-1202
Notas: ArticleID:2.7.1189
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ark:/67375/HXZ-0XR8HPD8-J
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: RNA extracted from CsCl.purified virions of tobacco mosaic virus is shown to give rise to an unusual nucleotide on digestion with RNAase T2, in addition to the four major nucleotides. This minor component has the electrophoretic characteristics of a phosphorylated end group, but is partially resistant to bacterial alkaline phosphatase. It is, however, a substrate for venom phos-phodiesterase or nucleotide pyrophosphatase, yielding products which imply the structure m7G5′ppp5′Gp. TMV RNA, like many animal cellular and viral mRNAs recently examined, therefore has a 5′ terminus blocked by a methylated nucleotide inverted with respect to the rest of the chain.
Editor: England: Oxford University Press
Idioma: Inglês

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The 5′ end group of tobacco mosaic virus RNA is m7G5′ppp5′Gp

Zimmern, D

England: Oxford University Press

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