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HIV-1 cellular and tissue replication patterns in infected humanized mice

Araínga, Mariluz ; Su, Hang ; Poluektova, Larisa Y ; Gorantla, Santhi ; Gendelman, Howard E

Scientific reports, 2016-03, Vol.6 (1), p.23513-23513, Article 23513 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
HIV-1 cellular and tissue replication patterns in infected humanized mice
Autor: Araínga, Mariluz ; Su, Hang ; Poluektova, Larisa Y ; Gorantla, Santhi ; Gendelman, Howard E
Assuntos: Acquired immune deficiency syndrome ; AIDS ; Allografts ; Animal models ; Animals ; Antigens, CD34 - metabolism ; Bone marrow ; Bone Marrow - virology ; Brain - virology ; CD14 antigen ; CD16 antigen ; CD34 antigen ; CD4-Positive T-Lymphocytes - virology ; Dendritic Cells - virology ; Deoxyribonucleic acid ; Digestive tract ; Disease Models, Animal ; DNA ; Effector cells ; Flow cytometry ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells - virology ; Hemopoiesis ; HIV Infections - virology ; HIV-1 - pathogenicity ; HIV-1 - physiology ; Humans ; Immunological memory ; Infections ; Kidney - virology ; Kidney transplantation ; Life cycles ; Liver ; Liver - virology ; Lung - virology ; Lymphocytes T ; Macrophages ; Macrophages - virology ; Memory cells ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Monocytes ; Monocytes - virology ; Replication ; Ribonucleic acid ; RNA ; Spleen - virology ; Stem cells ; Viral infections ; Virus Replication
É parte de: Scientific reports, 2016-03, Vol.6 (1), p.23513-23513, Article 23513
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Humanized mice have emerged as a testing platform for HIV-1 pathobiology by reflecting natural human disease processes. Their use to study HIV-1 biology, virology, immunology, pathogenesis and therapeutic development has served as a robust alternative to more-well developed animal models for HIV/AIDS. A critical component in reflecting such human pathobiology rests in defining the tissue and cellular sites for HIV-1 infection. To this end, we examined the tissue sites for viral infection in bone marrow, blood, spleens, liver, gut, brain, kidney and lungs of human CD34+ hematopoietic stem cell engrafted virus-infected NOD.Cg-Prkdc(scid) Il2rg(tm1Wjl)/SzJ mice. Cells were analyzed by flow cytometry and sorted from species mixtures defined as CD34+ lineage negative progenitor cells, CD14+CD16+ monocyte-macrophages and central, stem cell and effector memory T cells. The cell distribution and viral life cycle were found dependent on the tissue compartment and time of infection. Cell subsets contained HIV-1 total and integrated DNA as well as multi-spliced and unspliced RNA in divergent proportions. The data support the idea that humanized mice can provide a means to examine the multifaceted sites of HIV-1 replication including, but not limited to progenitor cells and monocyte-macrophages previously possible only in macaques and human.
Editor: England: Nature Publishing Group
Idioma: Inglês

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HIV-1 cellular and tissue replication patterns in infected humanized mice

Araínga, Mariluz ; Su, Hang ; Poluektova, Larisa Y ; Gorantla, Santhi ; Gendelman, Howard E

England: Nature Publishing Group

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