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CIL: 50699, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 3.3_Cell_3_Stack_Filtered. In Cell Image Library

Ivanchenko, Maryna V. ; Cicconet, Marcelo ; Jandal, Hoor Al ; Wu, Xudong ; Corey, David P. ; Indzhykulian, Artur A.

UC San Diego Library Digital Collections 2022

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  • Título:
    CIL: 50699, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 3.3_Cell_3_Stack_Filtered. In Cell Image Library
  • Autor: Ivanchenko, Maryna V. ; Cicconet, Marcelo ; Jandal, Hoor Al ; Wu, Xudong ; Corey, David P. ; Indzhykulian, Artur A.
  • Assuntos: Cuticular plate ; Hair cell stereocilia ; Inner ear outer hair cell ; mitochondria ; Mus musculus ; Native auditory Hair Cells at postnatal day 4 ; Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)
  • Notas: 10.7295/w9cil50699
    Imaging mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) Visualization methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads
    https://www.nature.com/articles/s41467-019-11712-w
    10.6075/j0r20zxn
    RelationTypeNote: IsPartOf -- 10.6075/j0r20zxn
    Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. 3.3_Cell_3_Stack_Filtered is a multipage TIFF file (2.6×2.6×15 nm³ voxel size) filtered with mean shift filter (kernel: 3D, 3 > max distance X & Y: 3 > noise spread: 1.0), unsharp filter (unit: 8 > kernel: 3D, 3 > standard deviation: 1.0 > unsharp factor: 1.0) and histogram balancing filter (set to reference image #170). This file is a part of Dataset 3, comprised of files related to the Cell #3 of current study. The numbering order of the files reflects the order of operations performed during the analysis.
    https://www.nature.com/articles/s41597-020-0509-4
    RelationTypeNote: IsDerivedFrom -- 10.7295/w9cil50699
  • Descrição: FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
  • Editor: UC San Diego Library Digital Collections
  • Data de criação/publicação: 2022
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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