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Refined human artificial chromosome vectors for gene therapy and animal transgenesis

KAZUKI, Y ; HOSHIYA, H ; UENO, E ; KAJITANI, N ; YOSHINO, T ; KAZUKI, K ; ISHIHARA, C ; TAKEHARA, S ; TSUJI, S ; EJIMA, F ; TOYODA, A ; SAKAKI, Y ; TAKIGUCHI, M ; LARIONOV, V ; KOUPRINA, N ; OSHIMURA, M ; ABE, S ; IIDA, Y ; OSAKI, M ; KATOH, M ; HIRATSUKA, M ; SHIRAYOSHI, Y ; HIRAMATSU, K

Gene therapy, 2011-04, Vol.18 (4), p.384-393 [Periódico revisado por pares]

London: Nature Publishing Group

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Título:
Refined human artificial chromosome vectors for gene therapy and animal transgenesis
Autor: KAZUKI, Y ; HOSHIYA, H ; UENO, E ; KAJITANI, N ; YOSHINO, T ; KAZUKI, K ; ISHIHARA, C ; TAKEHARA, S ; TSUJI, S ; EJIMA, F ; TOYODA, A ; SAKAKI, Y ; TAKIGUCHI, M ; LARIONOV, V ; KOUPRINA, N ; OSHIMURA, M ; ABE, S ; IIDA, Y ; OSAKI, M ; KATOH, M ; HIRATSUKA, M ; SHIRAYOSHI, Y ; HIRAMATSU, K
Assuntos: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Applied cell therapy and gene therapy ; Biological and medical sciences ; Biotechnology ; Cell Line ; Chromosomes ; Chromosomes, Artificial, Human ; Chromosomes, Human, Pair 21 ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene therapy ; Gene Transfer Techniques ; Genetic research ; Genetic Therapy - methods ; Genetic Vectors ; Health aspects ; Health. Pharmaceutical industry ; Herpes simplex virus ; Humans ; Industrial applications and implications. Economical aspects ; Medical sciences ; Mice ; Original ; Polymerase chain reaction ; Recombination, Genetic ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Transgenic animals
É parte de: Gene therapy, 2011-04, Vol.18 (4), p.384-393
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Current address: Comparative Genomics Laboratory, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan.
Current address: Toyohashi University of Technology, 1-1, Hibarigaoka, Tenpaku-cho, Toyohashi, Aichi 441-8580, Japan.
Descrição: Human artificial chromosomes (HACs) have several advantages as gene therapy vectors, including stable episomal maintenance, and the ability to carry large gene inserts. We previously developed HAC vectors from the normal human chromosomes using a chromosome engineering technique. However, endogenous genes were remained in these HACs, limiting their therapeutic applications. In this study, we refined a HAC vector without endogenous genes from human chromosome 21 in homologous recombination-proficient chicken DT40 cells. The HAC was physically characterized using a transformation-associated recombination (TAR) cloning strategy followed by sequencing of TAR-bacterial artificial chromosome clones. No endogenous genes were remained in the HAC. We demonstrated that any desired gene can be cloned into the HAC using the Cre-loxP system in Chinese hamster ovary cells, or a homologous recombination system in DT40 cells. The HAC can be efficiently transferred to other type of cells including mouse ES cells via microcell-mediated chromosome transfer. The transferred HAC was stably maintained in vitro and in vivo. Furthermore, tumor cells containing a HAC carrying the suicide gene, herpes simplex virus thymidine kinase (HSV-TK), were selectively killed by ganciclovir in vitro and in vivo. Thus, this novel HAC vector may be useful not only for gene and cell therapy, but also for animal transgenesis.
Editor: London: Nature Publishing Group
Idioma: Inglês

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Refined human artificial chromosome vectors for gene therapy and animal transgenesis

KAZUKI, Y ; HOSHIYA, H ; UENO, E ; KAJITANI, N ; YOSHINO, T ; KAZUKI, K ; ISHIHARA, C ; TAKEHARA, S ; TSUJI, S ; EJIMA, F ; TOYODA, A ; SAKAKI, Y ; TAKIGUCHI, M ; LARIONOV, V ; KOUPRINA, N ; OSHIMURA, M ; ABE, S ; IIDA, Y ; OSAKI, M ; KATOH, M ; HIRATSUKA, M ; SHIRAYOSHI, Y ; HIRAMATSU, K

London: Nature Publishing Group

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