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Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains

Médici, K C ; Barry, A F ; Alfieri, A F ; Alfieri, A A

Brazilian journal of medical and biological research, 2010-01, Vol.43 (1), p.13-16 [Periódico revisado por pares]

Brazil: Associação Brasileira de Divulgação Científica

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Título:
Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains
Autor: Médici, K C ; Barry, A F ; Alfieri, A F ; Alfieri, A A
Assuntos: Animals ; Base Sequence ; BIOLOGY ; Brazil ; Diarrhea ; Electrophoresis, Polyacrylamide Gel ; Feces - virology ; Genotype ; Group B rotavirus ; Humans ; MEDICINE, RESEARCH & EXPERIMENTAL ; Molecular Sequence Data ; NSP2 gene ; Phylogenetic analysis ; Phylogeny ; Piglets ; Polymerase Chain Reaction - methods ; RNA-Binding Proteins - genetics ; Rotavirus - classification ; Rotavirus - genetics ; Rotavirus - isolation & purification ; Swine ; Viral Nonstructural Proteins - genetics
É parte de: Brazilian journal of medical and biological research, 2010-01, Vol.43 (1), p.13-16
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Group B rotaviruses (RV-B) were first identified in piglet feces, being later associated with diarrhea in humans, cattle, lambs, and rats. In human beings, the virus was only described in China, India, and Bangladesh, especially infecting adults. Only a few studies concerning molecular analysis of the RV-B NSP2 gene have been conducted, and porcine RV-B has not been characterized. In the present study, three porcine wild-type RV-B strains from piglet stool samples collected from Brazilian pig herds were used for analysis. PAGE results were inconclusive for those samples, but specific amplicons of the RV-B NSP2 gene (segment 8) were obtained in a semi-nested PCR assay. The three porcine RV-B strains showed the highest nucleotide identity with the human WH1 strain and the alignments with other published sequences resulted in three groups of strains divided according to host species. The group of human strains showed 92.4 to 99.7% nucleotide identity while the porcine strains of the Brazilian RV-B group showed 90.4 to 91.8% identity to each other. The identity of the Brazilian porcine RV-B strains with outer sequences consisting of group A and C rotaviruses was only 35.3 to 38.8%. A dendrogram was also constructed to group the strains into clusters according to host species: human, rat, and a distinct third cluster consisting exclusively of the Brazilian porcine RV-B strains. This is the first study of the porcine RV-B NSP2 gene that contributes to the partial characterization of this virus and demonstrates the relationship among RV-B strains from different host species.
Editor: Brazil: Associação Brasileira de Divulgação Científica
Idioma: Inglês;Português

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Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains

Médici, K C ; Barry, A F ; Alfieri, A F ; Alfieri, A A

Brazil: Associação Brasileira de Divulgação Científica

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