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Intracellular glutathione (GSH) levels modulate mercuric chloride (MC)- and methylmercuric chloride (MeHgCl)-induced amino acid release from neonatal rat primary astrocytes cultures

Aschner, M. ; Mullaney, K.J. ; Wagoner, D. ; Lash, L.H. ; Kimelberg, H.K.

Brain research, 1994-11, Vol.664 (1), p.133-140 [Periódico revisado por pares]

London: Elsevier B.V

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Título:
Intracellular glutathione (GSH) levels modulate mercuric chloride (MC)- and methylmercuric chloride (MeHgCl)-induced amino acid release from neonatal rat primary astrocytes cultures
Autor: Aschner, M. ; Mullaney, K.J. ; Wagoner, D. ; Lash, L.H. ; Kimelberg, H.K.
Assuntos: Animals ; Animals, Newborn ; Aspartate taurine ; Aspartic Acid - metabolism ; Astrocyte ; Astrocytes - drug effects ; Astrocytes - metabolism ; Biological and medical sciences ; Buthionine Sulfoximine ; Cells, Cultured ; Chemical and industrial products toxicology. Toxic occupational diseases ; Glutamate-Cysteine Ligase - antagonists & inhibitors ; Glutathione ; Glutathione - metabolism ; Medical sciences ; Mercuric chloride ; Mercuric Chloride - pharmacology ; Metals and various inorganic compounds ; Methionine Sulfoximine - analogs & derivatives ; Methionine Sulfoximine - pharmacology ; Methylmercury ; Methylmercury Compounds - pharmacology ; Pyrrolidonecarboxylic Acid ; Rat ; Rats ; Rats, Sprague-Dawley ; Stimulation, Chemical ; Taurine - metabolism ; Thiazoles - pharmacology ; Thiazolidines ; Toxicology ; Tritium
É parte de: Brain research, 1994-11, Vol.664 (1), p.133-140
Notas: ObjectType-Article-2
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Descrição: Mercuric chloride (MC) and methylmercury (MeHg) were found to increase amino acid release from astrocytes. This suggests interaction with sulfhydryl (-SH) groups which are controlled by glutathione [GSH] levels. In the present study, we evaluated the effects of alterations in intracellular glutathione concentrations [GSH] i on the outcome of MC and MeHg treatment. [GSH] i were increased in a time-dependent fashion by incubating the astrocytes with 1 mM L-2-oxothiazolidine-4-carboxylic acid (OTC), a cysteine precursor. OTC attenuated the release of [2,3- 3H] D-aspartic acid from astrocytes exposed to MC-(5 μM) and MeHg-(10 μM). MeHg-induced [ 3H] D-taurine release was also reduced by pretreatment of astrocytes with OTC. Treatment with BSO (50 μM) decreased [GSH] i in astrocytes, and increased [2,3- 3H] D-aspartate release from MC- and MeHg-treated astrocytes, and [ 3H] D-taurine release from MeHg-treated cells. Neither OTC nor BSO when added to cultures in the absence of MC or MeHg had an effect on amino acid release by astrocytes. The current study underscores both the sensitivity of astrocytes to mercurials in terms of amino acid release and the relationship of these effects to astrocytic [GSH] i.
Editor: London: Elsevier B.V
Idioma: Inglês

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Intracellular glutathione (GSH) levels modulate mercuric chloride (MC)- and methylmercuric chloride (MeHgCl)-induced amino acid release from neonatal rat primary astrocytes cultures

Aschner, M. ; Mullaney, K.J. ; Wagoner, D. ; Lash, L.H. ; Kimelberg, H.K.

London: Elsevier B.V

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