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Interaction of phospholipid scramblase 1 with the Epstein-Barr virus protein BZLF1 represses BZLF1-mediated lytic gene transcription

Kusano, Shuichi ; Ikeda, Masanori

The Journal of biological chemistry, 2019-10, Vol.294 (41), p.15104-15116 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Interaction of phospholipid scramblase 1 with the Epstein-Barr virus protein BZLF1 represses BZLF1-mediated lytic gene transcription
Autor: Kusano, Shuichi ; Ikeda, Masanori
Assuntos: Active Transport, Cell Nucleus ; Antigens, Viral - genetics ; BZLF1 ; Cell Line, Tumor ; Cell Nucleus - metabolism ; Epstein-Barr virus ; Gene Expression Regulation ; herpesvirus ; Herpesvirus 4, Human - genetics ; Herpesvirus 4, Human - metabolism ; Herpesvirus 4, Human - physiology ; Humans ; Microbiology ; phospholipid scramblase 1 ; Phospholipid Transfer Proteins - metabolism ; Protein Binding ; protein-protein interaction ; Trans-Activators - metabolism ; transcription factor ; transcription regulation ; Transcription, Genetic ; viral transcription
É parte de: The Journal of biological chemistry, 2019-10, Vol.294 (41), p.15104-15116
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by Charles E. Samuel
Descrição: Human phospholipid scramblase 1 (PLSCR1) is strongly expressed in response to interferon (IFN) treatment and viral infection, and PLSCR1 has been suggested to play an important role in IFN-dependent antiviral responses. In this study, we showed that the basal expression of PLSCR1 was significantly elevated in Epstein-Barr virus (EBV)–infected nasopharyngeal carcinoma (NPC). PLSCR1 was observed to directly interact with the EBV immediate-early transactivator BZLF1 in vitro and in vivo, and this interaction repressed the BZLF1-mediated transactivation of an EBV lytic BMRF1 promoter construct. In addition, PLSCR1 expression decreased the BZLF1-mediated up-regulation of lytic BMRF1 mRNA and protein expression in WT and PLSCR1-knockout EBV-infected NPC cells. Furthermore, we showed that PLSCR1 represses the interaction between BZLF1 and CREB-binding protein (CBP), which enhances the BZLF1-mediated transactivation of EBV lytic promoters. These results reveal for the first time that PLSCR1 specifically interacts with BZLF1 and negatively regulates its transcriptional regulatory activity by preventing the formation of the BZLF1-CBP complex. This interaction may contribute to the establishment of latent EBV infection in EBV-infected NPC cells.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Interaction of phospholipid scramblase 1 with the Epstein-Barr virus protein BZLF1 represses BZLF1-mediated lytic gene transcription

Kusano, Shuichi ; Ikeda, Masanori

United States: Elsevier Inc

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